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Comparison of a direct antigen enzyme immunoassay, Herpchek, with cell culture for detection of herpes simplex virus from clinical specimens.直接抗原酶免疫测定法(Herpchek)与细胞培养法用于从临床标本中检测单纯疱疹病毒的比较。
J Clin Microbiol. 1995 May;33(5):1378-9. doi: 10.1128/jcm.33.5.1378-1379.1995.
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Herpes simplex virus detection from genital lesions: a comparative study using antigen detection (HerpChek) and culture.生殖器病变中单纯疱疹病毒的检测:一项使用抗原检测(HerpChek)和培养法的比较研究。
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J Clin Microbiol. 1989 Aug;27(8):1903-5. doi: 10.1128/jcm.27.8.1903-1905.1989.
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Light microscopy, culture, molecular, and serologic methods for detection of herpes simplex virus.用于检测单纯疱疹病毒的光学显微镜检查、培养、分子和血清学方法。
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Human monoclonal antibody fragment specific for glycoprotein G in herpes simplex virus type 2 with applications for serotype-specific diagnosis.针对2型单纯疱疹病毒糖蛋白G的人源单克隆抗体片段及其在血清型特异性诊断中的应用
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6
Typing of clinical herpes simplex virus type 1 and type 2 isolates with monoclonal antibodies.利用单克隆抗体对临床1型和2型单纯疱疹病毒分离株进行分型
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本文引用的文献

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Spin-amplified culture followed by enzyme immunoassay for detection of herpes simplex virus in patient specimens: a comparative study.旋转扩增培养后酶免疫测定法检测患者标本中的单纯疱疹病毒:一项比较研究。
Clin Diagn Virol. 1993 Mar;1(1):23-8. doi: 10.1016/0928-0197(93)90030-9.
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Herpes simplex virus detection from genital lesions: a comparative study using antigen detection (HerpChek) and culture.生殖器病变中单纯疱疹病毒的检测:一项使用抗原检测(HerpChek)和培养法的比较研究。
J Clin Microbiol. 1993 Jul;31(7):1774-6. doi: 10.1128/jcm.31.7.1774-1776.1993.
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Rapid detection of herpes simplex virus (HSV) antigen in human ocular infections.人类眼部感染中单纯疱疹病毒(HSV)抗原的快速检测
Curr Eye Res. 1988 Jul;7(7):661-6. doi: 10.3109/02713688809033194.
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Replacement of special enzyme immunoassay transport medium by a standard viral transport medium in the Herpcheck herpes simplex virus antigen detection test.在Herpcheck单纯疱疹病毒抗原检测试验中,用标准病毒运输培养基替代特殊酶免疫测定运输培养基。
Diagn Microbiol Infect Dis. 1989 Nov-Dec;12(6):473-5. doi: 10.1016/0732-8893(89)90080-1.
5
Evaluation of the Du Pont HERPCHEK herpes simplex virus antigen test with clinical specimens.使用临床标本对杜邦HERPCHEK单纯疱疹病毒抗原检测进行评估。
J Clin Microbiol. 1989 Aug;27(8):1903-5. doi: 10.1128/jcm.27.8.1903-1905.1989.
6
Diagnosis of herpes simplex virus infection in a clinical setting by a direct antigen detection enzyme immunoassay kit.使用直接抗原检测酶免疫分析试剂盒在临床环境中诊断单纯疱疹病毒感染。
J Clin Microbiol. 1989 Apr;27(4):700-4. doi: 10.1128/jcm.27.4.700-704.1989.
7
Herpes simplex virus antigen direct detection in standard virus transport medium by Du Pont Herpchek enzyme-linked immunosorbent assay.采用杜邦疱疹病毒检测酶联免疫吸附试验在标准病毒运输培养基中直接检测单纯疱疹病毒抗原。
J Clin Microbiol. 1990 Nov;28(11):2555-8. doi: 10.1128/jcm.28.11.2555-2558.1990.
8
Multicenter clinical evaluation of the Du Pont Herpchek HSV ELISA, a new rapid diagnostic test for the direct detection of herpes simplex virus.杜邦疱疹病毒酶联免疫吸附测定法(Du Pont Herpchek HSV ELISA)的多中心临床评估,这是一种用于直接检测单纯疱疹病毒的新型快速诊断测试。
Adv Exp Med Biol. 1990;263:71-6. doi: 10.1007/978-1-4613-0601-6_6.
9
Comparison of two enzyme-linked immunosorbent assays for detection of herpes simplex virus antigen.两种用于检测单纯疱疹病毒抗原的酶联免疫吸附测定法的比较
J Clin Microbiol. 1991 Mar;29(3):436-8. doi: 10.1128/jcm.29.3.436-438.1991.
10
Underdiagnosis of genital herpes by current clinical and viral-isolation procedures.当前临床及病毒分离程序对生殖器疱疹的诊断不足。
N Engl J Med. 1992 Jun 4;326(23):1533-9. doi: 10.1056/NEJM199206043262305.

直接抗原酶免疫测定法(Herpchek)与细胞培养法用于从临床标本中检测单纯疱疹病毒的比较。

Comparison of a direct antigen enzyme immunoassay, Herpchek, with cell culture for detection of herpes simplex virus from clinical specimens.

作者信息

Verano L, Michalski F J

机构信息

Diagnostic Virology Laboratory, Corning Clinical Laboratory, Teterboro, New Jersey 07608, USA.

出版信息

J Clin Microbiol. 1995 May;33(5):1378-9. doi: 10.1128/jcm.33.5.1378-1379.1995.

DOI:10.1128/jcm.33.5.1378-1379.1995
PMID:7615760
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228171/
Abstract

Direct enzyme immunoassay (Herpchek) was compared with culture on 21,522 specimens mainly from asymptomatic women for herpes simplex virus detection. Sensitivity and specificity were 73.8 and 97.7%, respectively. The 33% detection rate by enzyme immunoassay in 5 h increased to 43% when the enzyme immunoassay was combined with culture. Herpchek alone was not sensitive enough, but in combination with culture, maximum detection was achieved.

摘要

将直接酶免疫测定法(Herpchek)与培养法对主要来自无症状女性的21522份标本进行单纯疱疹病毒检测的结果进行了比较。敏感性和特异性分别为73.8%和97.7%。酶免疫测定法在5小时内的检出率为33%,当酶免疫测定法与培养法联合使用时,检出率提高到43%。单独使用Herpchek不够灵敏,但与培养法联合使用时,可实现最大检出率。