Nishikawa K, Matsuo S, Tamai H, Okada N, Okada H
Third Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan.
Immunology. 1998 Oct;95(2):302-7. doi: 10.1046/j.1365-2567.1998.00592.x.
MCA44 is a monoclonal antibody (mAb) to guinea-pig decay-accelerating factor (DAF) and, using this mAb, tissue distribution of guinea-pig DAF was studied by immunofluorescence. Guinea-pig DAF was found to be expressed not only on the vascular endothelium but also on different types of cells, such as the tubular epithelium of the kidney, epidermal cells of the skin and synovial lining cells. As there was no significant reduction in staining intensity with MCA44 following treatment with phosphatidylinositol-specific phospholipase C, many guinea-pig DAF molecules expressed in these tissues may be of the transmembrane form.
MCA44是一种针对豚鼠衰变加速因子（DAF）的单克隆抗体（mAb），利用该单克隆抗体，通过免疫荧光研究了豚鼠DAF的组织分布。发现豚鼠DAF不仅在血管内皮细胞上表达，还在不同类型的细胞上表达，如肾的肾小管上皮细胞、皮肤的表皮细胞和滑膜衬里细胞。由于用磷脂酰肌醇特异性磷脂酶C处理后，MCA44的染色强度没有显著降低，这些组织中表达的许多豚鼠DAF分子可能是跨膜形式。
