McAdory B S, Van Eldik L J, Norden J J
Department of Cell Biology, Medical Center North C-2310, Vanderbilt University Medical School, Nashville, TN 37232, USA.
Brain Res. 1998 Nov 30;813(1):211-7. doi: 10.1016/s0006-8993(98)01014-2.
Using light and electron microscopic immunocytochemistry, we examined the expression of the Ca2+-binding protein S100B in the dentate gyrus of adult rats during lesion-induced sprouting and reactive synaptogenesis. Nine days following unilateral lesioning of the entorhinal cortex, S100B was upregulated in cells primarily in the outer part of the molecular layer of the ipsilateral dentate gyrus. When examined with electron microscopy, numerous astrocytes and synapses containing S100B were identified. These data show that during lesion-induced sprouting and reactive synaptogenesis, S100B is upregulated in astrocytes and can be found in pre- and post-synaptic compartments where it might influence neuronal protein phosphorylation.
利用光镜和电镜免疫细胞化学技术，我们检测了成年大鼠齿状回在损伤诱导的轴突发芽和反应性突触形成过程中钙结合蛋白S100B的表达。在内嗅皮质单侧损伤九天后，S100B在同侧齿状回分子层外侧的主要细胞中上调。用电镜检查时，发现了许多含有S100B的星形胶质细胞和突触。这些数据表明，在损伤诱导的轴突发芽和反应性突触形成过程中，S100B在星形胶质细胞中上调，并且可以在突触前和突触后区室中发现，在那里它可能影响神经元蛋白磷酸化。
