Alexander J S, Dayton T, Davis C, Hill S, Jackson T H, Blaschuk O, Symonds M, Okayama N, Kevil C G, Laroux F S, Berney S M, Kimpel D
Louisiana State University Medical Center, Shreveport 71130, USA.
Inflammation. 1998 Dec;22(6):573-82. doi: 10.1023/a:1022310429868.
T-lymphocytes routinely traffic from the lymphoid and vascular compartments to the tissues during immune surveillance and inflammatory responses. This egress occurs without compromising endothelial barrier, which is maintained by tight junctions (zonula occludens). We report that T-lymphocytes up-regulate the expression of occludin, a major component of the tight junction in response to stimulation with phorbol ester (PMA) + calcium ionophore, CD3 antibody or T-cell receptor (TCR) antibody. Only activated T-lymphocytes express occludin; this adhesion molecule is nearly absent in resting T-lymphocytes. By immunofluorescence, occludin is seen in lymphocyte aggregates, but does not appear to mediate aggregation since only 50% of the cells in these clusters express occludin. Occludin is expressed between 8 and 24 h following stimulation, and persists for at least 48 h. These data indicate that activated T cells produce occludin which may regulate lymphocyte adhesion and trafficking.
在免疫监视和炎症反应期间，T淋巴细胞通常从淋巴和血管腔室迁移至组织。这种迁移发生时不会损害由紧密连接（闭锁小带）维持的内皮屏障。我们报告称，T淋巴细胞在受到佛波酯（PMA）+钙离子载体、CD3抗体或T细胞受体（TCR）抗体刺激后，会上调紧密连接的主要成分闭合蛋白的表达。只有活化的T淋巴细胞表达闭合蛋白；这种黏附分子在静息T淋巴细胞中几乎不存在。通过免疫荧光观察，闭合蛋白可见于淋巴细胞聚集体中，但似乎并不介导聚集，因为这些聚集体中只有50%的细胞表达闭合蛋白。闭合蛋白在刺激后8至24小时表达，并持续至少48小时。这些数据表明，活化的T细胞产生闭合蛋白，其可能调节淋巴细胞的黏附和迁移。
