Plasmans C M, Kuypers W, Slooff T J
Clin Orthop Relat Res. 1978 May(132):233-43.
The effect of diphosphonates (EHDP) was studied on induced ectopic osteoneogenesis. Decalcified allogeneic bone matrix was implanted into the lumbar muscles in rabbits for an ectopic osteoinductor. The ectopic new bone formation was studied under continuous EHDP administration and after discontinuation of EHDP. Although there was no inhibitory effect of EHDP on the osteoinduction, the calcification of the new bone and the resorption of the implanted matrix was retarded. The induced "osteoid-like" tissue had an atypical structure with a small number of cells and a deficiency in collagen fiber bundles. There were also large fields of new cartilage formation. Thus remodelling and mineralization were inhibited under the influence of continuous administration of EHDP. After discontinuation of EHDP administration a recovery phase was observed which seemed to lead to the formation of a normal ossicle.
研究了双膦酸盐（EHDP）对诱导性异位骨生成的影响。将脱钙同种异体骨基质植入兔腰部肌肉作为异位骨诱导剂。在持续给予EHDP以及停止给予EHDP后，研究异位新骨形成情况。尽管EHDP对骨诱导没有抑制作用，但新骨的钙化和植入基质的吸收受到了延迟。诱导产生的“类骨质样”组织结构不典型，细胞数量少且胶原纤维束缺乏。还存在大片新软骨形成区域。因此，在持续给予EHDP的影响下，重塑和矿化受到抑制。停止给予EHDP后，观察到一个恢复期，这似乎导致了正常小骨的形成。
