Okada Y, Saika S, Shirai K, Hashizume N, Yamanaka O, Ohnishi Y, Senba E
Department of Ophthalmology, Wakayama Medical College, Japan.
Graefes Arch Clin Exp Ophthalmol. 1998 Nov;236(11):853-8. doi: 10.1007/s004170050170.
We examined the immunolocalization of proto-oncogene products, including c-Fos and c-Jun, in the rat cornea during epithelial and stromal wound healing after simple epithelial ablation, penetrating injury or alkali burn.
Eighty-four male Wistar rats were divided into three groups and subjected to treatments as follows: (a) ablation of central corneal epithelium leaving basement membrane intact, (b) alkali burn in the central cornea with 1 N NaOH and (c) penetrating injury at the central cornea. The affected eyes were then enucleated after various intervals of healing. The frozen sections were immunohistochemically stained with the antibodies against proto-oncogene products.
c-Fos- and c-Jun-immunoreactive cells were detected in the epithelium around the epithelial defect from 60 to 120 min after these treatments. The distribution of these cells were varied in these three types of injury. The immunoreactivities for these proteins were also detected in keratocytes after epithelial ablation. In the corneas with alkali burn, the immunoreactivities were detected in the keratocytes in the whole corneal stroma, and these immunoreactions were stronger than those observed in simple epithelial ablation. In the corneas with penetrating injury, such immunoreaction was seen only in keratocytes around the wound.
These findings indicate that activator protein 1-mediated transcriptional activation for epithelial migration is initiated in the early phase after each injury, and that stromal keratocytes are also transcriptionally activated not only by alkali burn or penetrating injury but also by simple epithelial ablation in which basement membrane was not affected.
我们研究了原癌基因产物，包括c-Fos和c-Jun，在大鼠角膜单纯上皮切除、穿透伤或碱烧伤后上皮和基质伤口愈合过程中的免疫定位。
84只雄性Wistar大鼠分为三组，进行如下处理：(a)切除中央角膜上皮，保留基底膜完整；(b)用1 N NaOH对中央角膜进行碱烧伤；(c)对中央角膜进行穿透伤。然后在不同愈合时间间隔后摘除患眼。冰冻切片用抗原癌基因产物的抗体进行免疫组织化学染色。
在这些处理后60至120分钟，在上皮缺损周围的上皮中检测到c-Fos和c-Jun免疫反应性细胞。这三种损伤类型中这些细胞的分布有所不同。上皮切除后在角膜细胞中也检测到这些蛋白质的免疫反应性。在碱烧伤的角膜中，在整个角膜基质的角膜细胞中检测到免疫反应性，且这些免疫反应比单纯上皮切除中观察到的更强。在穿透伤的角膜中，这种免疫反应仅在伤口周围的角膜细胞中可见。
这些发现表明，在每次损伤后的早期阶段就启动了激活蛋白1介导的上皮迁移转录激活，并且基质角膜细胞不仅受到碱烧伤或穿透伤的转录激活，还受到基底膜未受影响的单纯上皮切除的转录激活。
