Khan M M, Muzammil S, Kumar Y, Tayyab S
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Uttar Pradesh, India.
J Biochem Biophys Methods. 1998 Sep 24;37(1-2):47-52. doi: 10.1016/s0165-022x(98)00017-7.
Serum albumin, when incubated with bilirubin prior to electrophoresis, was visualized as a yellow-colored band during the electrophoretic run and did not require any staining. Furthermore, free bilirubin served as a tracking dye. A minimum of 20 micrograms of protein was detected very well by this method. The formation of a bilirubin-albumin complex did not affect the electrophoretic mobility as the protein complexed with bilirubin as well as free albumin moved with the same mobility. Only a single protein band was visualized by this method after electrophoresis of human plasma. The method is simple, less time-consuming and may be used in identifying bilirubin-binding proteins in various biological samples.
血清白蛋白在电泳前与胆红素一起孵育时，在电泳过程中呈现为一条黄色条带，无需任何染色。此外，游离胆红素可作为示踪染料。用这种方法能很好地检测到至少20微克的蛋白质。胆红素 - 白蛋白复合物的形成并不影响电泳迁移率，因为与胆红素结合的蛋白质以及游离白蛋白以相同的迁移率移动。人血浆电泳后用这种方法仅可见一条蛋白质条带。该方法简单、耗时少，可用于鉴定各种生物样品中的胆红素结合蛋白。
