Putilina T, Jaworski C, Gentleman S, McDonald B, Kadiri M, Wong P
Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, Maryland, USA.
Biochem Biophys Res Commun. 1998 Nov 18;252(2):433-9. doi: 10.1006/bbrc.1998.9656.
A unique clone, isolated from a human pancreatic cDNA library, was sequenced and characterized. Northern blot analysis showed that the gene is active in a number of fetal and adult tissues, and immunoblots showed expression in nuclear and cytosolic cell fractions. The gene corresponding to the clone was localized to chromosome 13 by human/rodent somatic cell hybrid panels. The largest open reading frame contains a LIM domain, and the deduced peptide from the open reading frame appears to have the characteristics of a LIM-only protein, designated LMO7. RT-PCR and genomic sequence analyses indicate that expression of this gene product is subject to tissue-specific modulation by elimination of the LIM domain by alternative splicing in neural tissues.
