Mizutani M, Hashidoko Y, Tahara S
Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
FEBS Lett. 1998 Nov 6;438(3):236-40. doi: 10.1016/s0014-5793(98)01308-8.
In a survey of plant secondary metabolites regulating the behaviour of Aphanomyces cochlioides zoospores, we found that root extracts of Portulaca oleracea inhibited zoospore motility. Bioassay-directed fractionation of Portulaca constituents revealed that the inhibitory activity was dependent on the interaction of two chemically different factors. These were identified as a phenolic compound, N-trans-feruloyltyramine, which by itself was active as a zoospore stimulant, and an acidic compound, 1-linoleoyl-2-lysophosphatidic acid monomethyl ester, which had zoospore-repellent activity. When Chromosorb W AW particles coated with a mixture of these pure compounds were bioassayed in Petri dishes, the inhibitory effect on zoospore motility was identical with that caused by root tip or root extracts of P. oleracea. Inhibited zoospores rapidly settled to the bottom of the Petri dishes where they initially encysted, and then germinated within 1-2 h. This is the first report of factors which inhibit zoospore motility without killing or bursting the zoospores.
在一项关于调节嗜根腐霉游动孢子行为的植物次生代谢产物的调查中，我们发现马齿苋的根提取物能抑制游动孢子的运动。对马齿苋成分进行生物测定导向的分级分离后发现，抑制活性取决于两种化学性质不同的因子之间的相互作用。这两种因子被鉴定为一种酚类化合物N - 反式阿魏酰酪胺，其本身作为游动孢子刺激剂具有活性，以及一种酸性化合物1 - 亚油酰 - 2 - 溶血磷脂酸单甲酯，其具有游动孢子排斥活性。当用这些纯化合物的混合物包被的Chromosorb W AW颗粒在培养皿中进行生物测定时，对游动孢子运动的抑制作用与马齿苋根尖或根提取物所引起的抑制作用相同。受到抑制的游动孢子迅速沉降到培养皿底部，它们最初在那里形成包囊，然后在1 - 2小时内萌发。这是关于在不杀死或胀破游动孢子的情况下抑制游动孢子运动的因子的首次报道。
