Mansour S J, Herbrick J A, Scherer S W, Melançon P
Department of Cell Biology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada.
Genomics. 1998 Dec 1;54(2):323-7. doi: 10.1006/geno.1998.5563.
We present the cDNA sequence of human GBF1 along with data on expression pattern and genomic attributes. The deduced polypeptide encodes a putative guanine nucleotide exchange factor of 206.5 kDa, containing a centrally positioned Sec7 domain and a proline-rich region at the extreme C terminus. Its mRNA transcripts are found in 17 tissues and cell lines, likely suggesting a housekeeping role for GBF1p. The gene maps to 10q24, 2.33 cR from D10S540. It is fully contained within YAC 822C1 and covers at most 450 kb. Its Sec7 domain-encoding region harbors four introns.
我们展示了人类GBF1的cDNA序列以及关于表达模式和基因组特征的数据。推导的多肽编码一个推定的206.5 kDa鸟嘌呤核苷酸交换因子，其中心位置有一个Sec7结构域，在极端的C末端有一个富含脯氨酸的区域。其mRNA转录本在17种组织和细胞系中被发现，这可能表明GBF1p具有管家功能。该基因定位于10q24，距D10S540为2.33 cR。它完全包含在YAC 822C1中，最多覆盖450 kb。其Sec7结构域编码区域含有四个内含子。
