Paula-Lopes F F, de Moraes A A, Edwards J L, Justice J E, Hansen P J
a Department of Dairy&Poultry Sciences, University of Florida, Gainesville, Florida 32611-0920, USA.
Biol Reprod. 1998 Dec;59(6):1406-12. doi: 10.1095/biolreprod59.6.1406.
Experiments were performed to determine the actions of recombinant bovine interleukin-1beta (IL-1beta) on the growth of preimplantation embryos. In the first series of studies, IL-1beta was added at 8-10 h after insemination, and the percentage of oocytes developing to the blastocyst stage was evaluated. IL-1beta increased development to the blastocyst stage when embryos were cultured at high density ( approximately 25-30 embryos/drop) but decreased or had no effect on development when cultured at low density ( approximately 10 embryos/drop). Thus, the positive effect of IL-1beta depends upon some other embryo-derived product. The effect of IL-1beta on embryonic development was maintained in completely denuded embryos, indicating that cumulus cells do not mediate the actions of IL-1beta. Maximum development of embryos cultured at approximately 25-30/drop occurred at 0.1-1 ng/ml; 10 ng/ml was less effective. Addition of IL-1beta to groups of approximately 25-30 embryos/drop at 8-10 h after insemination also increased embryo cell number at Day 5 postinsemination by increasing the proportion of embryos that reached the 9- to 16-cell stage. However, IL-1beta had no effect on the proportion of blastocysts when added at Day 5 postinsemination. Thus, IL-1beta probably acts to increase blastocyst numbers by exerting actions on embryo growth before Day 5. In contrast to its effect on embryos, addition of IL-1beta during oocyte maturation did not affect cumulus expansion, cleavage rate of oocytes, or subsequent development to the blastocyst stage. In conclusion, IL-1beta can modulate growth of bovine embryos at early stages of development in a manner dependent upon embryo density.
进行实验以确定重组牛白细胞介素 -1β（IL -1β）对植入前胚胎生长的作用。在第一系列研究中，在授精后8 - 10小时添加IL -1β，并评估发育至囊胚阶段的卵母细胞百分比。当胚胎在高密度（约25 - 30个胚胎/滴）下培养时，IL -1β增加了发育至囊胚阶段的比例，但在低密度（约10个胚胎/滴）下培养时，其降低了发育比例或对发育没有影响。因此，IL -1β的积极作用取决于一些其他胚胎衍生产物。IL -1β对胚胎发育的影响在完全去除卵丘细胞的胚胎中仍然存在，这表明卵丘细胞不介导IL -1β的作用。在约25 - 30个/滴培养的胚胎中，最大发育发生在0.1 - 1 ng/ml；10 ng/ml的效果较差。在授精后8 - 10小时向约25 - 30个胚胎/滴的组中添加IL -1β，通过增加达到9至16细胞阶段的胚胎比例，也增加了授精后第5天的胚胎细胞数量。然而，在授精后第5天添加IL -1β时，对囊胚比例没有影响。因此，IL -1β可能通过在第5天之前对胚胎生长发挥作用来增加囊胚数量。与其对胚胎的作用相反，在卵母细胞成熟期间添加IL -1β不影响卵丘扩展、卵母细胞的分裂率或随后发育至囊胚阶段。总之，IL -1β可以以依赖于胚胎密度的方式调节牛胚胎发育早期的生长。
