Mills T M, Lewis R W, Stopper V S
Department of Physiology and Endocrinology, Medical College of Georgia, Augusta 30912-3000, USA.
Biol Reprod. 1998 Dec;59(6):1413-8. doi: 10.1095/biolreprod59.6.1413.
Ongoing studies in this laboratory are designed to determine the role of androgens in the maintenance of the erectile response in the rat. Testosterone-treated castrated rats (TESTO) and untreated castrated rats (CASTRATE) were used for measurement of the rate at which blood flows into the cavernous sinuses by timed collections of blood after partial amputation of the penis. A laser Doppler flow meter was employed to determine whether androgens also regulate the veno-occlusive mechanism that controls the rate of blood flow out of the sinuses. Erection was induced by direct electrical stimulation of the autonomic ganglion that controls cavernosal blood flow in the erectile response. The results of these studies showed that blood flow into the sinuses was approximately twice as great in the TESTO animals as the CASTRATE rats. Furthermore, during ganglionic stimulation, veno-occlusion occurred in the TESTO rats but failed to occur in the CASTRATE rats. The dependence of these responses on nitric oxide (NO) was demonstrated by showing that injection of sodium nitroprusside (SNP) enhances the intracavernosal pressure response in TESTO rats but not CASTRATE animals. However, when SNP injection was combined with ganglionic stimulation, veno-occlusion did occur in the CASTRATE animals. Taken together, these studies show that both the rate of blood flow into the cavernous sinuses and the blood flow out are under androgenic regulation and may involve the actions of NO.
本实验室正在进行的研究旨在确定雄激素在维持大鼠勃起反应中的作用。用睾酮处理过的去势大鼠（TESTO）和未处理的去势大鼠（CASTRATE），在阴茎部分切除后通过定时采血来测量血液流入海绵窦的速率。使用激光多普勒流量计来确定雄激素是否也调节控制血液从窦中流出速率的静脉闭塞机制。通过直接电刺激控制勃起反应中海绵体血流的自主神经节来诱导勃起。这些研究结果表明，TESTO组动物流入窦中的血流量大约是CASTRATE组大鼠的两倍。此外，在神经节刺激期间，TESTO组大鼠发生了静脉闭塞，而CASTRATE组大鼠未发生。通过表明注射硝普钠（SNP）可增强TESTO组大鼠而非CASTRATE组动物的海绵体内压力反应，证明了这些反应对一氧化氮（NO）的依赖性。然而，当SNP注射与神经节刺激相结合时，CASTRATE组动物确实发生了静脉闭塞。综上所述，这些研究表明，流入海绵窦的血流速率和流出的血流均受雄激素调节，并且可能涉及NO的作用。
