Baselt D R, Lee G U, Natesan M, Metzger S W, Sheehan P E, Colton R J
Naval Research Laboratory, Washington DC 20375-5342, USA.
Biosens Bioelectron. 1998 Oct 1;13(7-8):731-9. doi: 10.1016/s0956-5663(98)00037-2.
We are developing a biosensor that will measure, at the level of single molecules, the forces that bind DNA-DNA, antibody-antigen, or ligand-receptor pairs together. The Bead Array Counter (BARC) will use these interaction forces to hold magnetic microbeads to a solid substrate. Microfabricated magnetoresistive transducers on the substrate will indicate whether or not the beads are removed when pulled by magnetic forces. By adapting magnetoresistive computer memory technology, it may be possible to fabricate millions of transducers on a chip and detect or screen thousands of analytes. The multi-analyte capability of this portable sensor would be ideal for on-site testing, while the potential to directly gauge intermolecular interaction strengths suggests drug discovery applications.
我们正在开发一种生物传感器，它能够在单分子水平上测量将DNA-DNA、抗体-抗原或配体-受体对结合在一起的力。珠阵列计数器（BARC）将利用这些相互作用力将磁性微珠固定在固体基质上。基质上的微加工磁阻传感器将指示微珠在受到磁力作用时是否被移除。通过采用磁阻计算机存储技术，有可能在芯片上制造数百万个传感器，并检测或筛选数千种分析物。这种便携式传感器的多分析物检测能力对于现场检测来说将是理想的，而直接测量分子间相互作用强度的潜力则暗示了其在药物发现方面的应用。
