Imhoff J F, Süling J, Petri R
Institut für Meereskunde, Universität Kiel, Abteilung Marine Mikrobiologie, Kiel, Germany.
Int J Syst Bacteriol. 1998 Oct;48 Pt 4:1129-43. doi: 10.1099/00207713-48-4-1129.
Sequences of the 16S rDNA from all available type strains of Chromatium species have been determined and were compared to those of other Chromatiaceae, a few selected Ectothiorhodospiraceae and Escherichia coli. The clear separation of Ectothiorhodospiraceae and Chromatiaceae is confirmed. Most significantly the sequence comparison revealed a genetic divergence between Chromatium species originated from freshwater sources and those of truly marine and halophilic nature. Major phylogenetic branches of the Chromatiaceae contain (i) marine and halophilic species, (ii) freshwater Chromatium species together with Thiocystis species and (iii) species of the genera Thiocapsa and Amoebobacter as recently reclassified [Guyoneaud, R. & 6 other authors (1988). Int J Syst Bacteriol 48, 957-964], namely Thiocapsa roseopersicina, Thiocapsa pendens (formerly Amoebobacter pendens), Thiocapsa rosea (formerly Amoebobacter roseus), Amoebobacter purpureus and Thiolamprovum pedioforme (formerly Amoebobacter pedioformis). The genetic relationships between the species and groups are not in congruence with the current classification of the Chromatiaceae and a reclassification is proposed on the basis of 16S rDNA sequence similarity supported by selected phenotypic properties. The proposed changes include the transfers of Chromatium minus and Chromatium violascens to Thiocystis minor comb. nov. and Thiocystis violascens comb. nov., of Chromatium vinosum, Chromatium minutissimum and Chromatium warmingii to the new genus Allochromatium as Allochromatium vinosum comb. nov., Allochromatium minutissimum comb. nov., and Allochromatium warmingii comb. nov., of Chromatium tepidum to the new genus Thermochromatium as Thermochromatium tepidum comb. nov., of Chromatium salexigens and Chromatium glycolicum to the new genus Halochromatium as Halochromatium salexigens comb. nov. and Halochromatium glycolicum comb. nov., of Chromatium gracile and Chromatium purpuratum to the new genus as Marichromatium gracile comb. nov. and Marichromatium purpuratum comb. nov., of Thiocapsa pfennigii to Thiococcus pfennigii gen. nom. rev., of Thiocapsa halophila to the new genus Thiohalocapsa as Thiohalocapsa halophila comb. nov., and of Chromatium buderi to the new genus Isochromatium as Isochromatium buderi comb. nov.
已测定了所有可用的嗜色菌属模式菌株的16S rDNA序列，并将其与其他着色菌科、一些选定的外硫红螺菌科以及大肠杆菌的序列进行了比较。外硫红螺菌科和着色菌科的明确区分得到了证实。最显著的是，序列比较揭示了源自淡水的嗜色菌属物种与真正的海洋和嗜盐性质的物种之间的遗传差异。着色菌科的主要系统发育分支包含：（i）海洋和嗜盐物种；（ii）淡水嗜色菌属物种以及囊硫菌属物种；（iii）硫小杆菌属和变形杆菌属的物种（如最近重新分类的那样）[Guyoneaud, R. 及其他6位作者（1988年）。《国际系统细菌学杂志》48, 957 - 964]，即玫瑰色硫小杆菌、悬垂硫小杆菌（原名悬垂变形杆菌）、玫瑰色硫小杆菌（原名玫瑰色变形杆菌）、紫色变形杆菌和蹼状硫灯菌（原名蹼状变形杆菌）。这些物种和类群之间的遗传关系与目前着色菌科的分类不一致，因此基于16S rDNA序列相似性并辅以选定的表型特征，提出了重新分类的建议。提议的变化包括：将微小嗜色菌和紫色嗜色菌转移至小囊硫菌，分别为新组合小囊硫菌微小变种和小囊硫菌紫色变种；将葡萄酒色嗜色菌、极小嗜色菌和瓦氏嗜色菌转移至新属异嗜色菌属，分别为新组合异嗜色菌葡萄酒色变种、异嗜色菌极小变种和异嗜色菌瓦氏变种；将温热嗜色菌转移至新属嗜热嗜色菌属，为新组合嗜热嗜色菌温热变种；将嗜盐嗜色菌和糖嗜色菌转移至新属嗜盐嗜色菌属，分别为新组合嗜盐嗜色菌嗜盐变种和嗜盐嗜色菌糖变种；将纤细嗜色菌和紫色嗜色菌转移至新属海嗜色菌属，分别为新组合海嗜色菌纤细变种和海嗜色菌紫色变种；将芬氏硫小杆菌转移至芬氏硫球菌，为名称修正；将嗜盐硫小杆菌转移至新属嗜盐硫小杆菌属，为新组合嗜盐硫小杆菌嗜盐变种；将布氏嗜色菌转移至新属等嗜色菌属，为新组合等嗜色菌布氏变种。
