Jain R, Malhotra V, Kumar N, Sarin S K
Department of Pathology, G.B. Pant Hospital, New Delhi, India.
Trop Gastroenterol. 1998 Jul-Sep;19(3):100-1.
The argyrophilic technique for staining proteins associated with nucleolar organizer regions (AgNOR's) was applied to 32 liver biopsy specimens. These included 10 normal livers, 10 cirrhosis, 10 hepatocellular carcinomas (HCC) and 2 cholangiocarcinomas. The range of the mean number of NORs per nucleus was 1.3-1.7 (95% CI: 1.4-1.6) for normal liver, 2.4-3.5 (2.8-3.2) for cirrhosis, 3.0-7.9 (3.7-5.6) for HCC and 4.0-4.2 (3.9-4.3) for cholangiocarcinoma. The difference observed was statistically significant. The AgNORs were small and discrete in normal and cirrhotic liver and relatively larger and scattered in distribution in HCC. These results indicate that both quantitative and qualitative analysis of AgNORs may be useful as an adjunct to routine haematoxylin and eosin stain to distinguish cirrhosis from HCC especially when the latter is well differentiated.
将用于染色与核仁组织区相关蛋白质的嗜银技术（AgNORs）应用于32例肝活检标本。这些标本包括10例正常肝脏、10例肝硬化、10例肝细胞癌（HCC）和2例胆管癌。正常肝脏每核NORs平均数范围为1.3 - 1.7（95%可信区间：1.4 - 1.6），肝硬化为2.4 - 3.5（2.8 - 3.2），HCC为3.0 - 7.9（3.7 - 5.6），胆管癌为4.0 - 4.2（3.9 - 4.3）。观察到的差异具有统计学意义。AgNORs在正常和肝硬化肝脏中较小且离散，而在HCC中相对较大且分布分散。这些结果表明，AgNORs的定量和定性分析作为常规苏木精和伊红染色的辅助手段，对于区分肝硬化和HCC可能是有用的，特别是当后者分化良好时。
