Kanitakis J, al-Rifai I, Faure M, Claudy A
Department of Dermatology, Hôpital Eduoard Herriot, Lyon, France.
J Clin Pathol. 1998 Aug;51(8):588-92. doi: 10.1136/jcp.51.8.588.
To study the immunohistochemical expression of the Thomsen-Friedenreich antigen (T) and its precursor, Tn, in the skin in various cancers.
T and Tn antigens were studied with monoclonal antibodies in 91 primary premalignant and malignant lesions, 13 cases of Paget's disease, and 26 carcinomas metastatic to the skin. The material had been collected over a 10 year period, formalin fixed, and paraffin embedded. Diagnoses had been made after examination of standard histological sections, supplemented when needed by appropriate immunohistochemical staining.
21% and 29% of the primary cutaneous premalignant and malignant epithelial tumours expressed the Tn and T antigens, respectively. By contrast, 81% of metastatic carcinomas to the skin were Tn positive, while only 23% of them expressed the T antigen. All cases of Paget's disease were Tn positive but only 15% of them expressed the T antigen. The 21 nonepithelial tumours (including melanomas) were as a rule unreactive.
The accumulation of the precursor (Tn) antigen in tumours metastasising to the skin highlights the incomplete glycosylation of carbohydrate antigens occurring in these tumours. The predominant Tn versus T antigen expression appears to be a useful immunohistochemical feature which may aid in the differentiation of primary cutaneous carcinomas from metastatic tumours.
研究汤姆森-弗里德赖希抗原（T）及其前体Tn在各种癌症皮肤中的免疫组化表达。
用单克隆抗体研究了91例原发性癌前病变和恶性病变、13例佩吉特病病例以及26例皮肤转移癌中的T和Tn抗原。这些材料是在10年期间收集的，经福尔马林固定并石蜡包埋。在检查标准组织切片后做出诊断，必要时辅以适当的免疫组化染色。
原发性皮肤癌前和恶性上皮肿瘤分别有21%和29%表达Tn和T抗原。相比之下，81%的皮肤转移癌Tn呈阳性，而其中只有23%表达T抗原。所有佩吉特病病例Tn均呈阳性，但只有15%表达T抗原。21例非上皮性肿瘤（包括黑色素瘤）通常无反应。
转移至皮肤的肿瘤中前体（Tn）抗原的积累突出了这些肿瘤中发生的碳水化合物抗原糖基化不完全的情况。Tn与T抗原的主要表达似乎是一种有用的免疫组化特征，可能有助于原发性皮肤癌与转移瘤的鉴别。
