Jones R R, Spaull J, Gusterson B
St John's Hospital for Diseases of the Skin, London, UK.
Histopathology. 1989 Apr;14(4):409-16. doi: 10.1111/j.1365-2559.1989.tb02169.x.
The histogenesis of mammary and extramammary Paget's disease has been studied by immunohistochemical staining of paraffin-embedded tissue using a panel of epithelial cell markers, which react with secretory or ductal epithelium, but not stratified epithelium. These markers included a monoclonal antibody E29 to epithelial membrane antigen EMA, the cytokeratin marker CAM 5.2 and three new monoclonal antibodies raised to human milk fat globule membrane (LICR-LON-TW19 and H.10.A) and a human bladder cell cancer line (3.77). The findings demonstrate that both mammary and extramammary Paget's disease are of epithelial cell origin and share antigens expressed by simple epithelia. Some antigens, such as EMA and low molecular weight cytokeratins are consistently present in both diseases, whereas other antigens, identified by H.10.A and TW19 are found more frequently in cases of extramammary Paget's disease. This panel of monoclonal antibodies also proved useful in distinguishing Paget's disease from pagetoid melanoma and clear cell Bowen's disease.
通过使用一组上皮细胞标志物对石蜡包埋组织进行免疫组织化学染色，研究了乳腺和乳腺外佩吉特病的组织发生。这些标志物可与分泌性或导管上皮反应，但不与复层上皮反应。这些标志物包括针对上皮膜抗原EMA的单克隆抗体E29、细胞角蛋白标志物CAM 5.2以及针对人乳脂肪球膜（LICR-LON-TW19和H.10.A）和人膀胱癌细胞系（3.77）产生的三种新的单克隆抗体。研究结果表明，乳腺和乳腺外佩吉特病均起源于上皮细胞，并共享由单层上皮表达的抗原。一些抗原，如EMA和低分子量细胞角蛋白，在两种疾病中均持续存在，而由H.10.A和TW19鉴定的其他抗原在乳腺外佩吉特病病例中更常见。这组单克隆抗体在区分佩吉特病与派杰样黑色素瘤和透明细胞鲍恩病方面也被证明是有用的。
