• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

荚膜红细菌中NH₄⁺对固氮酶活性的短期调控：体内固氮酶对添加NH₄⁺的多种响应

Short-term regulation of nitrogenase activity by NH4+ in Rhodobacter capsulatus: multiple in vivo nitrogenase responses to NH4+ addition.

作者信息

Yakunin A F, Hallenbeck P C

机构信息

Département de Microbiologie et Immunologie, Université de Montréal, Montréal, Québec H3C 3J7, Canada.

出版信息

J Bacteriol. 1998 Dec;180(23):6392-5. doi: 10.1128/JB.180.23.6392-6395.1998.

DOI:10.1128/JB.180.23.6392-6395.1998
PMID:9829952
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107729/
Abstract

The photosynthetic bacterium Rhodobacter capsulatus has been shown to carry out nitrogenase "switch-off," a rapid, reversible inhibition of in vivo activity. Here, we demonstrate that highly nitrogen-limited cultures of both the wild-type strain and a draT draG mutant are capable of nitrogenase switch-off while moderately nitrogen-limited cultures show instead a "magnitude" response, with a decrease in in vivo nitrogenase activity that is proportional to the amount of added NH4+.

摘要

光合细菌荚膜红细菌已被证明能进行固氮酶“关闭”，即对体内活性进行快速、可逆的抑制。在此，我们证明野生型菌株和draT draG突变体的高度氮限制培养物都能够进行固氮酶关闭，而中度氮限制培养物则表现出“量值”反应，体内固氮酶活性的降低与添加的NH4+量成比例。

相似文献

1
Short-term regulation of nitrogenase activity by NH4+ in Rhodobacter capsulatus: multiple in vivo nitrogenase responses to NH4+ addition.荚膜红细菌中NH₄⁺对固氮酶活性的短期调控：体内固氮酶对添加NH₄⁺的多种响应
J Bacteriol. 1998 Dec;180(23):6392-5. doi: 10.1128/JB.180.23.6392-6395.1998.
2
Regulation of nitrogenase in the photosynthetic bacterium Rhodobacter sphaeroides containing draTG and nifHDK genes from Rhodobacter capsulatus.含有来自荚膜红细菌的draTG和nifHDK基因的光合细菌球形红细菌中固氮酶的调控。
Can J Microbiol. 2001 Mar;47(3):206-12.
3
Mutations affecting nitrogenase switch-off in Rhodobacter capsulatus.影响荚膜红细菌中固氮酶关闭的突变
Biochim Biophys Acta. 1992 Jan 9;1118(2):161-8. doi: 10.1016/0167-4838(92)90145-4.
4
The draTG gene region of Rhodobacter capsulatus is required for post-translational regulation of both the molybdenum and the alternative nitrogenase.荚膜红细菌的draTG基因区域对于钼和替代固氮酶的翻译后调控都是必需的。
J Gen Microbiol. 1993 Nov;139(11):2667-75. doi: 10.1099/00221287-139-11-2667.
5
Regulation of nitrogenase activity in Rhodobacter capsulatus under dark microoxic conditions.黑暗微氧条件下荚膜红细菌中固氮酶活性的调控
Arch Microbiol. 2000 May-Jun;173(5-6):366-72. doi: 10.1007/s002030000156.
6
Effect of P(II) and its homolog GlnK on reversible ADP-ribosylation of dinitrogenase reductase by heterologous expression of the Rhodospirillum rubrum dinitrogenase reductase ADP-ribosyl transferase-dinitrogenase reductase-activating glycohydrolase regulatory system in Klebsiella pneumoniae.通过在肺炎克雷伯菌中异源表达红螺菌二氮还原酶 ADP-核糖基转移酶-二氮还原酶激活糖水解酶调节系统，研究 P(II) 及其同系物 GlnK 对二氮还原酶可逆 ADP-核糖基化的影响。
J Bacteriol. 2001 Mar;183(5):1610-20. doi: 10.1128/JB.183.5.1610-1620.2001.
7
Role of GlnB and GlnK in ammonium control of both nitrogenase systems in the phototrophic bacterium Rhodobacter capsulatus.谷氨酰胺结合蛋白B（GlnB）和谷氨酰胺结合蛋白K（GlnK）在光合细菌荚膜红假单胞菌两种固氮酶系统的铵控制中的作用。
Microbiology (Reading). 2003 Aug;149(Pt 8):2203-2212. doi: 10.1099/mic.0.26235-0.
8
Reversible ADP-ribosylation is demonstrated to be a regulatory mechanism in prokaryotes by heterologous expression.通过异源表达证明可逆ADP核糖基化是原核生物中的一种调节机制。
Proc Natl Acad Sci U S A. 1990 Mar;87(5):1720-4. doi: 10.1073/pnas.87.5.1720.
9
The presence of ADP-ribosylated Fe protein of nitrogenase in Rhodobacter capsulatus is correlated with cellular nitrogen status.荚膜红细菌中固氮酶的ADP核糖基化铁蛋白的存在与细胞氮状态相关。
J Bacteriol. 1999 Apr;181(7):1994-2000. doi: 10.1128/JB.181.7.1994-2000.1999.
10
Posttranslational modification of nitrogenase. Differences between the purple bacterium Rhodospirillum rubrum and the cyanobacterium Anabaena variabilis.固氮酶的翻译后修饰。紫色细菌深红红螺菌与多变鱼腥藻之间的差异。
Eur J Biochem. 1994 Feb 15;220(1):125-30. doi: 10.1111/j.1432-1033.1994.tb18606.x.

引用本文的文献

1
Enhanced Hydrogen Production by Co-cultures of Hydrogenase and Nitrogenase in Escherichia coli.大肠杆菌中氢化酶和固氮酶共培养提高产氢量
Curr Microbiol. 2016 Mar;72(3):242-7. doi: 10.1007/s00284-015-0941-4. Epub 2015 Nov 25.
2
Ammonia-induced formation of an AmtB-GlnK complex is not sufficient for nitrogenase regulation in the photosynthetic bacterium Rhodobacter capsulatus.氨诱导的AmtB - GlnK复合物的形成不足以调节光合细菌荚膜红细菌中的固氮酶。
J Bacteriol. 2008 Mar;190(5):1588-94. doi: 10.1128/JB.01643-07. Epub 2007 Dec 21.
3
Membrane sequestration of PII proteins and nitrogenase regulation in the photosynthetic bacterium Rhodobacter capsulatus.光合细菌荚膜红假单胞菌中PII蛋白的膜隔离与固氮酶调控
J Bacteriol. 2007 Aug;189(16):5850-9. doi: 10.1128/JB.00680-07. Epub 2007 Jun 22.
4
AmtB is necessary for NH(4)(+)-induced nitrogenase switch-off and ADP-ribosylation in Rhodobacter capsulatus.在荚膜红细菌中，AmtB对于铵离子（NH₄⁺）诱导的固氮酶关闭和ADP核糖基化是必需的。
J Bacteriol. 2002 Aug;184(15):4081-8. doi: 10.1128/JB.184.15.4081-4088.2002.
5
Distinct roles of P(II)-like signal transmitter proteins and amtB in regulation of nif gene expression, nitrogenase activity, and posttranslational modification of NifH in Azoarcus sp. strain BH72.类P(II)信号转导蛋白和amtB在调控偶氮弧菌属BH72菌株中nif基因表达、固氮酶活性及NifH翻译后修饰中的不同作用
J Bacteriol. 2002 Apr;184(8):2251-9. doi: 10.1128/JB.184.8.2251-2259.2002.
6
Response of the endophytic diazotroph Gluconacetobacter diazotrophicus on solid media to changes in atmospheric partial O(2) pressure.内生固氮菌葡糖醋杆菌在固体培养基上对大气中氧分压变化的响应。
Appl Environ Microbiol. 2001 Oct;67(10):4694-700. doi: 10.1128/AEM.67.10.4694-4700.2001.
7
Role of a ferredoxin gene cotranscribed with the nifHDK operon in N(2) fixation and nitrogenase "switch-off" of Azoarcus sp. strain BH72.与固氮酶操纵子共转录的铁氧化还原蛋白基因在偶氮弧菌属菌株BH72的固氮及固氮酶“关闭”过程中的作用
J Bacteriol. 2001 Jun;183(12):3752-60. doi: 10.1128/JB.183.12.3752-3760.2001.
8
P(II) signal transduction proteins, pivotal players in microbial nitrogen control.P(II) 信号转导蛋白，微生物氮控制中的关键参与者。
Microbiol Mol Biol Rev. 2001 Mar;65(1):80-105. doi: 10.1128/MMBR.65.1.80-105.2001.
9
Enhanced nitrogenase activity in strains of Rhodobacter capsulatus that overexpress the rnf genes.在过表达rnf基因的荚膜红细菌菌株中固氮酶活性增强。
J Bacteriol. 2000 Mar;182(5):1208-14. doi: 10.1128/JB.182.5.1208-1214.2000.
10
The presence of ADP-ribosylated Fe protein of nitrogenase in Rhodobacter capsulatus is correlated with cellular nitrogen status.荚膜红细菌中固氮酶的ADP核糖基化铁蛋白的存在与细胞氮状态相关。
J Bacteriol. 1999 Apr;181(7):1994-2000. doi: 10.1128/JB.181.7.1994-2000.1999.

本文引用的文献

1
A luminol/iodophenol chemiluminescent detection system for western immunoblots.
Anal Biochem. 1998 Apr 10;258(1):146-9. doi: 10.1006/abio.1998.2571.
2
Salmonella typhimurium apparently perceives external nitrogen limitation as internal glutamine limitation.鼠伤寒沙门氏菌显然将外部氮限制视为内部谷氨酰胺限制。
J Mol Biol. 1996 Jun 21;259(4):589-607. doi: 10.1006/jmbi.1996.0342.
3
Posttranslational regulation of nitrogenase in Rhodobacter capsulatus: existence of two independent regulatory effects of ammonium.荚膜红细菌中固氮酶的翻译后调控：铵存在两种独立的调控作用。
J Bacteriol. 1993 Mar;175(5):1358-66. doi: 10.1128/jb.175.5.1358-1366.1993.
4
The draTG gene region of Rhodobacter capsulatus is required for post-translational regulation of both the molybdenum and the alternative nitrogenase.荚膜红细菌的draTG基因区域对于钼和替代固氮酶的翻译后调控都是必需的。
J Gen Microbiol. 1993 Nov;139(11):2667-75. doi: 10.1099/00221287-139-11-2667.
5
Nitrogenase from the photosynthetic bacterium Rhodopseudomonas capsulata: purification and molecular properties.来自光合细菌荚膜红假单胞菌的固氮酶：纯化及分子特性
J Bacteriol. 1982 Feb;149(2):708-17. doi: 10.1128/jb.149.2.708-717.1982.
6
Inhibition of nitrogenase activity by NH+4 in Rhodospirillum rubrum.红螺菌中铵离子对固氮酶活性的抑制作用。
J Bacteriol. 1981 Feb;145(2):824-31. doi: 10.1128/jb.145.2.824-831.1981.
7
Uptake of methionine sulfoximine by some N2 fixing bacteria, and its effect on ammonium transport.一些固氮细菌对甲硫氨酸亚砜亚胺的摄取及其对铵转运的影响。
FEBS Lett. 1983 Nov 28;164(1):121-3. doi: 10.1016/0014-5793(83)80032-5.
8
Reversible regulation of the nitrogenase iron protein from Rhodospirillum rubrum by ADP-ribosylation in vitro.体外通过ADP-核糖基化对红螺菌固氮酶铁蛋白进行可逆调节。
J Bacteriol. 1986 May;166(2):513-8. doi: 10.1128/jb.166.2.513-518.1986.
9
Ammonium and methylammonium transport in Rhodobacter sphaeroides.球形红杆菌中铵和甲基铵的转运
J Bacteriol. 1987 Apr;169(4):1632-8. doi: 10.1128/jb.169.4.1632-1638.1987.
10
Glutamine synthetase from Escherichia coli.来自大肠杆菌的谷氨酰胺合成酶。
Methods Enzymol. 1985;113:213-41. doi: 10.1016/s0076-6879(85)13032-6.