Maillard J Y, Hann A C, Perrin R
Welsh School of Pharmacy, University of Wales, Cardiff, UK.
J Appl Microbiol. 1998 Nov;85(5):799-806. doi: 10.1046/j.1365-2672.1998.00578.x.
The development of viral resistance to sodium hypochlorite was investigated using the Pseudomonas aeruginosa bacteriophage F116 as a model system. This phage was chosen because of its structural characteristics and former investigations conducted in this laboratory. F116 was shown to be sensitive to a sodium hypochlorite concentration of 0.0075 gl-1 (available chlorine) which produced a 5 log10 reduction in titre in a suspension test. Survival bacteriophages challenged with this sodium hypochlorite concentration were isolated, propagated and challenged again with the same and higher concentrations of the biocide. It was observed that progeny virions were becoming increasingly resistant to sodium hypochlorite challenges up to a concentration of 0.0175 gl-1 of available chlorine. It was also noticed that 1-2 log10 of F116 virions from resistant phage lysates remained sensitive to the biocide. An electron microscopical investigation of F116 resistant lysates showed that the phage resistance to sodium hypochlorite was not caused by F116 particles aggregation. Furthermore, no morphological difference between the sensitive and resistant F116 particles to sodium hypochlorite was identified.
以铜绿假单胞菌噬菌体F116作为模型系统，研究了病毒对次氯酸钠的抗性发展。选择该噬菌体是基于其结构特征以及本实验室之前进行的研究。结果表明，F116对0.0075 gl-1（有效氯）的次氯酸钠浓度敏感，在悬浮试验中该浓度可使噬菌体滴度降低5个对数级。分离出经此浓度次氯酸钠攻击后存活的噬菌体，进行传代培养，然后用相同浓度及更高浓度的杀菌剂再次攻击。结果发现，子代病毒粒子对次氯酸钠攻击的抗性不断增强，直至有效氯浓度达到0.0175 gl-1。还注意到，来自抗性噬菌体裂解物的1-2个对数级的F116病毒粒子对该杀菌剂仍敏感。对F116抗性裂解物进行电子显微镜研究表明，噬菌体对次氯酸钠的抗性并非由F116颗粒聚集所致。此外，未发现对次氯酸钠敏感和抗性的F116颗粒在形态上存在差异。
