Gilkerson J R, Love D N, Drummer H E, Studdert M J, Whalley J M
Department of Veterinary Anatomy and Pathology, University of Sydney, New South Wales.
Aust Vet J. 1998 Oct;76(10):677-82. doi: 10.1111/j.1751-0813.1998.tb12282.x.
To investigate the seroprevalence of equine herpesvirus 1 in foals around weaning and after weaning on two large Thoroughbred farms using a type-specific enzyme-linked immunosorbent assay to determine exposure to infection.
A longitudinal population study in groups of Thoroughbred weanling foals.
Two hundred weanling Thoroughbred foals from a population of about 380 foals were enrolled on two adjacent stud farms in the Hunter Valley of New South Wales. Foals on both farms were weaned from February to May 1995 into randomly selected groups of 10 to 15 foals. Farms were selected because of their willingness to cooperate in the survey and because their detailed records of foals and their movements. They were representative of well-managed large Thoroughbred stud farms in New South Wales. Both studs had upper respiratory tract disease among weanling foals around weaning each year although the seroprevalence of viral respiratory disease on either farm was not known before the study.
Serum was collected from foals within each group at fortnightly intervals from 9th February until 1st June 1995, and at a single follow-up period in August 1995. Each sample was tested in triplicate using an antibody-detection ELISA which is type-specific for EHV-1 and EHV-4 antibodies.
There was serological evidence of EHV-1 infection both before and after weaning. The prevalence of EHV-1 antibody in the sample population increased during the study and individual cases of EHV-1 infection were identified. The increase was caused both by the seroconversion of foals within the groups and by the recruitment into the study of foals with pre-existing EHV-1 antibody. Evidence of EHV-1 infection in Thoroughbred foals after weaning has not been reported previously in Australia and this has implications for vaccination regimens.
使用型特异性酶联免疫吸附测定法，调查两个大型纯种马养殖场断奶前后马驹中1型马疱疹病毒的血清流行率，以确定是否感染过该病毒。
对纯种断奶幼驹群体进行纵向研究。
从新南威尔士州猎人谷两个相邻种马场约380匹幼驹中选取200匹断奶纯种马驹。两个马场的马驹于1995年2月至5月断奶，随机分为每组10至15匹。选择这两个马场是因为它们愿意配合调查，且有幼驹及其活动的详细记录。它们代表了新南威尔士州管理良好的大型纯种马种马场。每年断奶前后，两个马场的断奶幼驹都有上呼吸道疾病，不过在研究之前，两个马场病毒性呼吸道疾病的血清流行率均未知。
从1995年2月9日至6月1日，每隔两周从每组马驹中采集血清，并于1995年8月进行一次随访。每个样本使用针对EHV-1和EHV-4抗体的型特异性抗体检测酶联免疫吸附测定法进行三次检测。
断奶前后均有血清学证据表明存在EHV-1感染。在研究期间，样本群体中EHV-1抗体的流行率有所上升，并鉴定出个别EHV-1感染病例。这种上升是由组内马驹的血清转化以及研究中纳入已有EHV-1抗体的马驹共同导致的。澳大利亚此前尚未报道过断奶后纯种马驹感染EHV-1的证据，这对疫苗接种方案有影响。
