Fernández H, Toro J
Instituto de Microbiología Clínica, Universidad Austral de Chile, Valdivia, Chile.
Rev Med Chil. 1998 Aug;126(8):919-23.
Small intestine alterations produced by the enterotoxigenic capacity of Campylobacter jejuni subsp. jejuni are similar to the hydric, electrolytic and pathological changes caused by choleraic and thermolabile Escherichia coli toxins.
To study the enterotoxigenic capacity of 4 strains of Campylobacter jejuni subsp. jejuni using the intestinal loop model.
Rat intestinal loops were inoculated with culture filtrates of the four strains. Enterotoxigenicity was assessed by fluid accumulation, the increase in Na+ and Cl- in the loop fluid, and cAMP increase in loop tissues. An enterotoxigenic Escherichia coil strain and sterile Brucella both were used as positive and negative controls, respectively.
The filtrates of two strains produced fluid accumulation in the loops, significantly increased Na+ and Cl- secretion to the intestinal lumen and increased tissue cAMP levels.
Some strains of Campylobacter jejuni subsp. jejuni are able to show enterotoxigenicity in vivo, increasing cAMP levels in the intestinal cells and altering electrolyte exchange mechanisms.
空肠弯曲菌空肠亚种的产肠毒素能力所导致的小肠改变类似于霍乱毒素和不耐热大肠杆菌毒素引起的水、电解质及病理变化。
使用肠袢模型研究4株空肠弯曲菌空肠亚种的产肠毒素能力。
将4株菌的培养滤液接种到大鼠肠袢中。通过液体蓄积、肠袢液中Na⁺和Cl⁻的增加以及肠袢组织中cAMP的增加来评估产肠毒素性。分别使用产肠毒素的大肠杆菌菌株和无菌布鲁氏菌作为阳性和阴性对照。
两株菌的滤液使肠袢出现液体蓄积，显著增加了向肠腔的Na⁺和Cl⁻分泌，并提高了组织cAMP水平。
一些空肠弯曲菌空肠亚种菌株能够在体内表现出产肠毒素性，增加肠道细胞中的cAMP水平并改变电解质交换机制。
