Gleispach H
Wien Klin Wochenschr. 1976 Apr 16;88(8):261-4.
The most important chemical pathological investigations which require to be undertaken in the emergency laboratory of a paediatric hospital are of the serum electrolytes, blood glucose, cerebrospinal fluid glucose, blood urea nitrogen, total proteins, bilirubin and calcium. It is imperative to have the opportunity of controlling these parameters both during the day and night. In order to be independent of the presence of a technician we were interested in finding an instrument which can be used by untrained personnel and gives results of sufficient accuracy. Hence, the clinicard analyser was tested in regard to reproducibility and accuracy of results when used by highly-trained technicians and by persons without knowledge of laboratory work. It was established that the values obtained by these two groups are similar and sufficiently reproducible for emergency use. The accuracy of the instrument was tested with 2 charges of cuvettes and with different control sera. In the case of total proteins differences were found between the different control sera used. The values obtained for blood urea nitrogen and for blood glucose lay within the range given for Labtrol and for Seronorm. The accuracy of bilirubin was tested with 5 different control sera and all values lay within the ranged given by the factory. Calcium was tested with Labtrol and Fluinorm and also with sera and urines from different patients. The values were within the range given for the test sera and were comparable to those measured with the calcium analyser of EEL, but were about 7% lower than those determined using atomic absorption. The values obtained for cerebrospinal fluid glucose correlated well with those obtained by the routine method.
在儿科医院的急诊实验室需要进行的最重要的化学病理检查项目包括血清电解质、血糖、脑脊液葡萄糖、血尿素氮、总蛋白、胆红素和钙。必须有机会在白天和晚上都能对这些参数进行检测。为了不依赖技术人员的在场,我们希望找到一种未经培训的人员也能使用且结果具有足够准确性的仪器。因此,对临床分析仪在由训练有素的技术人员以及不具备实验室工作知识的人员使用时的结果重现性和准确性进行了测试。结果表明,这两组人员获得的值相似,且对于急诊使用而言具有足够的可重复性。使用两批比色杯以及不同的对照血清对该仪器的准确性进行了测试。在总蛋白方面,发现所使用的不同对照血清之间存在差异。血尿素氮和血糖所获得的值在Labtrol和Seronorm给出的范围内。用5种不同的对照血清对胆红素的准确性进行了测试,所有值均在厂家给出的范围内。用Labtrol和Fluinorm以及不同患者的血清和尿液对钙进行了测试。这些值在测试血清给出的范围内,与用EEL钙分析仪测得的值相当,但比用原子吸收法测定的值低约7%。脑脊液葡萄糖所获得的值与常规方法测得的值相关性良好。