通过定点诱变产生核糖核酸酶抗体酶

Creation of a ribonuclease abzyme through site-directed mutagenesis.

作者信息

Fletcher M C, Kuderova A, Cygler M, Lee J S

机构信息

Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.

出版信息

Nat Biotechnol. 1998 Nov;16(11):1065-7. doi: 10.1038/3519.

DOI:10.1038/3519

PMID:9831037

Abstract

The development of abzymes (antibody/enzymes) is one method of creating reagents with novel catalytic activity. To date, most abzymes have been obtained by immunization with transition state analogs. We have chosen to start with an existing antibody and convert it into an enzyme by the addition of catalytic residues to the binding site. We have introduced a histidine residue into antibody Jel 103 and converted it into an abzyme that cleaves poly(rI) with a kinetic efficiency of about 100 M(-1) sec(-1).

摘要

抗体酶（抗体/酶）的开发是一种创造具有新型催化活性试剂的方法。迄今为止，大多数抗体酶是通过用过渡态类似物进行免疫获得的。我们选择从一种现有的抗体开始，通过在结合位点添加催化残基将其转化为酶。我们已将一个组氨酸残基引入抗体Jel 103，并将其转化为一种抗体酶，该抗体酶切割聚（rI）的动力学效率约为100 M⁻¹ s⁻¹。