Chapman F, Visvanathan K, Carreño-Manjarrez R, Zabriskie J B
Laboratory of Clinical Microbiology and Immunology, The Rockefeller University, New York, NY 10021, USA.
J Immunol Methods. 1998 Oct 1;219(1-2):181-6. doi: 10.1016/s0022-1759(98)00141-0.
D8/17 is proving to be an important diagnostic and treatment tool in patients with Tourette's syndrome (TS) and obsessive compulsive disorder (OCD). The present study represents a direct comparison of immunofluorescence microscopy and flow cytometry in terms of their relative efficiency in the detection of the DB/17 B cell marker. Analysis of 41 patients with OCD or TS and 31 control subjects by immunofluorescence microscopy and flow cytometer demonstrated an average of 6.5% of D8/17-positive B cells in controls with an average of 19.6% in OCD patients. Using the flow cytometer, values of 6.2% for controls and 22.4% for OCD patients were obtained. The flow cytometer test was able to count many more cells, induce less operator error and offer faster analysis while correlating strongly with the microscopic technique. Overall in our sample, the FACS assay test has a sensitivity of 77% and a specificity of 87%. The ease of use and close correlation to disease activity makes this an ideal test for clinical laboratories.
事实证明，D8/17是抽动秽语综合征（TS）和强迫症（OCD）患者重要的诊断和治疗工具。本研究直接比较了免疫荧光显微镜和流式细胞术检测DB/17 B细胞标志物的相对效率。通过免疫荧光显微镜和流式细胞仪对41例强迫症或抽动秽语综合征患者及31名对照者进行分析，结果显示对照组中平均有6.5%的D8/17阳性B细胞，强迫症患者中平均为19.6%。使用流式细胞仪检测，对照组的值为6.2%，强迫症患者为22.4%。流式细胞仪检测能够计数更多细胞，减少操作人员误差，提供更快的分析，同时与显微镜技术高度相关。总体而言，在我们的样本中，流式细胞仪检测的灵敏度为77%，特异性为87%。其易用性以及与疾病活动的密切相关性使其成为临床实验室的理想检测方法。
