• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Determination of trans-beta-carotene and other carotenoids in blood plasma using high-performance liquid chromatography and thermal lens detection.

作者信息

Franko M, van de Bovenkamp P, Bicanic D

机构信息

School of Environmental Sciences, Nova Gorica, Slovenia.

出版信息

J Chromatogr B Biomed Sci Appl. 1998 Oct 23;718(1):47-54. doi: 10.1016/s0378-4347(98)00347-8.

DOI:10.1016/s0378-4347(98)00347-8
PMID:9832359
Abstract

Thermal lens spectrometry (TLS) was applied for the detection of beta-cryptoxanthin, alpha-carotene, trans-beta-carotene, and lycopen in blood plasma. This combined high-performance liquid chromatography-TLS (HPLC-TLS) method was validated by comparison with HPLC-UV-Vis analysis of blood plasma under identical chromatographic conditions and by comparing the results obtained from an independent, standard HPLC procedure for determination of carotenoids in blood plasma samples. The results demonstrated good agreement with the target values for carotenoids in an in-laboratory control sample and confirmed the accuracy of the HPLC-TLS technique. Limits of detection for blood plasma samples were 70 pg/ml for beta-cryptoxanthin, 85 pg/ml for alpha-carotene, 100 pg/ml for trans-beta-carotene, and 120 pg/ml for lycopen. This represents a 100-fold improvement compared to the HPLC analysis with UV-Vis detection.

摘要

相似文献

1
Determination of trans-beta-carotene and other carotenoids in blood plasma using high-performance liquid chromatography and thermal lens detection.
J Chromatogr B Biomed Sci Appl. 1998 Oct 23;718(1):47-54. doi: 10.1016/s0378-4347(98)00347-8.
2
An improved HPLC method for determination of carotenoids in human serum.一种用于测定人血清中类胡萝卜素的改进高效液相色谱法。
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Sep 25;824(1-2):99-106. doi: 10.1016/j.jchromb.2005.07.004.
3
Comparison of high-performance liquid chromatography/tandem mass spectrometry and high-performance liquid chromatography/photo-diode array detection for the quantitation of carotenoids, retinyl esters, α-tocopherol and phylloquinone in chylomicron-rich fractions of human plasma.高效液相色谱/串联质谱法与高效液相色谱/光电二极管阵列检测法比较分析人富含乳糜微粒血浆中类胡萝卜素、视黄醇酯、α-生育酚和叶绿醌的含量。
Rapid Commun Mass Spectrom. 2013 Jun 30;27(12):1393-402. doi: 10.1002/rcm.6576.
4
Determination of lycopene, alpha-carotene and beta-carotene in serum by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry with selected-ion monitoring.
J Chromatogr B Biomed Sci Appl. 1998 Apr 24;708(1-2):67-73. doi: 10.1016/s0378-4347(97)00669-5.
5
Retinol, alpha-tocopherol, lutein/zeaxanthin, beta-cryptoxanthin, lycopene, alpha-carotene, trans-beta-carotene, and four retinyl esters in serum determined simultaneously by reversed-phase HPLC with multiwavelength detection.采用反相高效液相色谱法结合多波长检测同时测定血清中的视黄醇、α-生育酚、叶黄素/玉米黄质、β-隐黄质、番茄红素、α-胡萝卜素、反式-β-胡萝卜素以及四种视黄酯。
Clin Chem. 1994 Mar;40(3):411-6.
6
Consumption of canned citrus fruit meals increases human plasma β-cryptoxanthin concentration, whereas lycopene and β-carotene concentrations did not change in healthy adults.食用罐装柑橘类水果餐会增加健康成年人血浆中β-隐黄质的浓度，而番茄红素和β-胡萝卜素的浓度则没有变化。
Nutr Res. 2016 Jul;36(7):679-88. doi: 10.1016/j.nutres.2016.03.005. Epub 2016 Mar 18.
7
Determination of carotenoids in vegetable foods and plasma.蔬菜类食物和血浆中类胡萝卜素的测定。
Int J Vitam Nutr Res. 1997;67(1):47-54.
8
Determination of lycopene and beta-carotene by high-performance liquid chromatography using sudan I as internal standard.以苏丹红I为内标物，采用高效液相色谱法测定番茄红素和β-胡萝卜素。
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jun 21;838(1):44-9. doi: 10.1016/j.jchromb.2006.04.004. Epub 2006 May 22.
9
A routine method for the simultaneous measurement of retinol, alpha-tocopherol and five carotenoids in human plasma by reverse phase HPLC.一种通过反相高效液相色谱法同时测定人血浆中视黄醇、α-生育酚和五种类胡萝卜素的常规方法。
Clin Chim Acta. 1998 Feb 23;270(2):85-100. doi: 10.1016/s0009-8981(97)00224-6.
10
Plasma carotenoids in German children and adolescents.德国儿童和青少年的血浆类胡萝卜素
Eur J Pediatr. 1997 Jan;156(1):71-3. doi: 10.1007/s004310050557.

引用本文的文献

1
Accuracy of Measurements of Thermophysical Parameters by Dual-Beam Thermal-Lens Spectrometry.双光束热透镜光谱法测量热物理参数的准确性
Nanomaterials (Basel). 2023 Jan 20;13(3):430. doi: 10.3390/nano13030430.
2
Simultaneous analysis of circulating 25-hydroxy-vitamin D3, 25-hydroxy-vitamin D2, retinol, tocopherols, carotenoids, and oxidized and reduced coenzyme Q10 by high performance liquid chromatography with photo diode-array detection using C18 and C30 columns alone or in combination.同时分析循环 25-羟基维生素 D3、25-羟基维生素 D2、视黄醇、生育酚、类胡萝卜素、氧化和还原型辅酶 Q10，采用 C18 和 C30 柱单独或联合使用高效液相色谱法和光电二极管阵列检测。
J Chromatogr A. 2013 Aug 2;1301:1-9. doi: 10.1016/j.chroma.2013.05.027. Epub 2013 Jun 12.
3
Analysis of circulating lipid-phase micronutrients in humans by HPLC: review and overview of new developments.高效液相色谱法分析人体循环脂质相微量营养素：综述及新进展概述。
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jul 15;931:23-41. doi: 10.1016/j.jchromb.2013.04.031. Epub 2013 May 9.