Kanda T, Yokosuka O, Tagawa M, Kawai S, Imazeki F, Saisho H
First Department of Medicine, Chiba University School of Medicine, Japan.
J Hepatol. 1998 Nov;29(5):707-14. doi: 10.1016/s0168-8278(98)80250-x.
BACKGROUND/AIMS: Although GB virus C (GBV-C) is frequently detectable in patients with liver diseases, it is not known whether it actually replicates in the liver. Therefore we have quantitatively examined the strand-specific RNA of this virus.
Fourteen patients (two GBV-C RNA only seropositive, seven both GBV-C RNA and HCV RNA seropositive, and five HCV RNA only seropositive) were examined. Extracted RNAs from sera and liver specimens of these patients were serially diluted and strand-specific RNAs of GBV-C and HCV were quantitatively measured using strand-specific primers by reverse transcription-polymerase chain reaction.
Positive-strand GBV-C RNA in serum was detected in all nine GBV-C RNA seropositive cases, and negative-strand RNA was detected in three, uncertain in three, and undetected in three. Positive-strand GBV-C RNA in the liver was detected in seven and undetected in two, while negative-strand RNA in the liver was undetected in eight and uncertain in one. On the other hand, positive-strand HCV RNA was detected in serum from all 12 HCV RNA seropositive patients and negative-strand HCV RNA was detected in one, uncertain in seven, and undetected in four. Positive-strand HCV RNA was detected in the liver from all 12 HCV RNA seropositive patients, and the presence of negative-strand HCV RNA in the liver was confirmed in 10 and uncertain in the remaining two.
GBV-C is considered to be far less hepatotropic than HCV, and it is suggested that GBV-C might not be replicating in the liver.
背景/目的：尽管在肝病患者中经常能检测到GB病毒C（GBV-C），但尚不清楚它是否真的在肝脏中复制。因此，我们对该病毒的链特异性RNA进行了定量检测。
对14例患者进行了检测（2例仅GBV-C RNA血清学阳性，7例GBV-C RNA和HCV RNA均血清学阳性，5例仅HCV RNA血清学阳性）。对这些患者血清和肝脏标本中提取的RNA进行系列稀释，使用链特异性引物通过逆转录-聚合酶链反应对GBV-C和HCV的链特异性RNA进行定量测定。
在所有9例GBV-C RNA血清学阳性病例的血清中均检测到正链GBV-C RNA，3例检测到负链RNA，3例结果不确定，3例未检测到。7例肝脏中检测到正链GBV-C RNA，2例未检测到，而8例肝脏中未检测到负链RNA，1例结果不确定。另一方面，在所有12例HCV RNA血清学阳性患者的血清中均检测到正链HCV RNA，1例检测到负链HCV RNA，7例结果不确定，4例未检测到。12例HCV RNA血清学阳性患者的肝脏中均检测到正链HCV RNA，10例肝脏中确认存在负链HCV RNA，其余2例结果不确定。
GBV-C被认为比HCV的嗜肝性弱得多，提示GBV-C可能不在肝脏中复制。
