Clark D E, Smith S K, Licence D, Evans A L, Charnock-Jones D S
Reproductive Molecular Research Group, Department of Obstetrics and Gynaecology, University of Cambridge, Rosie Hospital, Cambridge, CB2 2SW, UK.
J Endocrinol. 1998 Dec;159(3):459-67. doi: 10.1677/joe.0.1590459.
Angiogenesis and vascular transformation are important processes in the normal development of the placenta. Vascular endothelial growth factor (VEGF) is a potent angiogenic growth factor and is thought to be important for placental development. Recently several new members of this family have been described. In this study we used in situ hybridisation to localise which cells in the placenta expressed mRNA for VEGF, placenta growth factor (PlGF), VEGF-B and VEGF-C. We were unable to find any message for either VEGF-B or VEGF-C in the placenta, suggesting that only low levels are produced which this method was unable to detect. The mRNA encoding VEGF was found to be produced by cells within the villous mesenchyme, decidual macrophages and decidual glands but, in contrast to our previous findings, not by trophoblast. The mRNA encoding PlGF was produced in large amounts by villous cytotrophoblast, syncytiotrophoblast and extravillous trophoblast. The mRNAs encoding VEGF and PlGF were thus not co-localised and it appears that there is unlikely to be any significant production of VEGF/PlGF heterodimer in the placenta.
血管生成和血管转化是胎盘正常发育过程中的重要过程。血管内皮生长因子（VEGF）是一种强大的血管生成生长因子，被认为对胎盘发育很重要。最近，这个家族的几个新成员已被描述。在本研究中，我们使用原位杂交来定位胎盘中哪些细胞表达VEGF、胎盘生长因子（PlGF）、VEGF-B和VEGF-C的mRNA。我们在胎盘中未能找到VEGF-B或VEGF-C的任何信息，这表明其产生水平很低，该方法无法检测到。发现编码VEGF的mRNA由绒毛间质细胞、蜕膜巨噬细胞和蜕膜腺细胞产生，但与我们之前的发现相反，滋养层细胞不产生。编码PlGF的mRNA由绒毛细胞滋养层、合体滋养层和绒毛外滋养层大量产生。因此，编码VEGF和PlGF的mRNA没有共定位，并且似乎胎盘中不太可能有任何显著的VEGF/PlGF异二聚体产生。
