Sampson H W, Spears H
Department of Human Anatomy and Neurobiology, College of Medicine, Texas A&M University, College Station 77843-1114, USA.
Alcohol Clin Exp Res. 1999 Feb;23(2):324-7.
Our project was conducted to determine if the deleterious effects of chronic alcohol consumption on growing bone are reversible if the adolescent stops drinking. Four-week old, female, Sprague-Dawley rats were housed and maintained in an AAALAC-accredited facility. Six animals each were placed on alcohol-fed (35% ethanol-derived calories), pair-fed or chow-fed diets for 2 or 4 weeks. A recovery group of six animals was alcohol-fed for 2 weeks followed by an additional 2 weeks of chow feeding. This group was pair-fed to an additional group of six animals that received liquid diet, pair fed to the recovery group for 2 weeks followed by 2 weeks on a pair-fed chow diet. Blood alcohol concentrations averaged 309 +/- 9 mg/dl. Morphological parameters of the femur, such as length, diameter, and volume were smaller in alcohol treated animals at both 2 and 4 weeks of feeding. Femur length and volume of recovery alcohol-fed animals were more than either 2- or 4-week alcohol-fed animals, but they were not as great as the same-age 4-week pair-fed or chow-fed animals. Diameter was similar to the 4-week alcohol-fed, but less than the chow-fed. Femur density was reduced at all time periods in the alcohol-fed animals. The recovery alcohol-fed animals had greater density than the 2-week alcohol, but not the 4-week alcohol-fed animals. They did not, however, reach 4-week chow- or pair-fed levels. Tibia BV/TV was reduced in the 2- and 4-week alcohol- and pair-fed animals. BV/TV was greater in the recovery animals than either 2- or 4-week alcohols, but not as great as the chow-fed animals. At 2 weeks, calorie deprivation caused a reduction in insulin-like growth factor-1 (IGF-1) that was reduced even more by alcohol. By 4 weeks, the calorie deprivation was no longer seen, but alcohol continued to reduce the values. Two weeks of alcohol followed by 2 weeks of chow diet returned the IGF-1 values to almost normal, but significantly different levels. The apparent improvement was probably due to continued growth of the young bones and not a regaining of bone lost during alcohol consumption.
我们开展该项目是为了确定，如果青少年停止饮酒，长期饮酒对生长中骨骼的有害影响是否可逆。四周龄的雌性斯普拉格-道利大鼠饲养于经实验动物评估与认可委员会（AAALAC）认证的设施中。每组六只动物，分别给予含酒精饲料（35%乙醇热量）、配对饲料或普通饲料，喂养2周或4周。另外有一组六只动物组成的恢复组，先给予酒精饲料喂养2周，随后再给予普通饲料喂养2周。该组与另一组同样六只动物进行配对喂养，这组动物接受流质饮食，与恢复组配对喂养2周，随后再给予配对的普通饲料喂养2周。血液酒精浓度平均为309±9毫克/分升。在喂养2周和4周时，接受酒精处理的动物股骨的形态学参数，如长度、直径和体积均较小。恢复组中接受酒精喂养的动物的股骨长度和体积比接受2周或4周酒精喂养的动物要大，但不如同龄的4周配对喂养或普通喂养的动物。直径与接受4周酒精喂养的动物相似，但小于普通喂养的动物。在所有时间段，接受酒精喂养的动物的股骨密度均降低。恢复组中接受酒精喂养的动物的密度比接受2周酒精喂养的动物要高，但比接受4周酒精喂养的动物低。然而，它们并未达到4周普通喂养或配对喂养动物的水平。在接受2周和4周酒精及配对饲料喂养的动物中，胫骨骨体积分数（BV/TV）降低。恢复组动物的BV/TV比接受2周或4周酒精喂养的动物要高，但不如普通喂养的动物。在2周时，热量剥夺导致胰岛素样生长因子-1（IGF-1）减少，而酒精使其减少得更多。到4周时，不再出现热量剥夺，但酒精继续使其值降低。先给予2周酒精喂养，随后给予2周普通饲料喂养，IGF-1值几乎恢复正常，但仍存在显著差异。这种明显的改善可能是由于幼骨的持续生长，而非恢复了饮酒期间丢失的骨骼。
