Meissner W
Zentralbl Bakteriol Orig B. 1976 Jul;162(3-4):330-49.
Two experiments carried out with one hundred rats from the Osborne-Mendel-strain were supposed to prove the influence of selenium-containing-drinking-water on caries. The animals were divided into five experimental groups of twenty rats each. Selenium was tested in different doses as an addition to drinking-water in shape of Na2SeO4, as well as in combination with a tooth-paste containing NaF.-Its toxicity was to be determined by the water consumption, the mortality and above all by the body weight of the animals at the end of the test. During the first experiment three groups received Na2SeO4 in doses of 1, 5 and 10 ppm in the drinking water and the cariogenic diet Stephan 580 as food. Two other groups served as control, one receiving the cariogenic diet Stephan 580 and the other one getting the normal breeding diet. The analysis of variance carried out for the assessment of caries and the test of SCHEFFE only show a different attack concerning those groups, receiving the cariogenic diet and the control group fed with the breeding diet Altromin R. Nine of the 40 animals of the groups that had received the selenium doses of 5 and 10 ppm did not survive the experiment. The average value of the water consumption and the body weight of the animals of the selenium group was below the average of the remaining groups, which points out a strong toxicity of Na2SeO4. In regard to the water consumptions, however, one cannot exactly define, whether it is an effect of the selenium of the cariogenic diet. All groups of the second experiment received the cariogenic diet Stephan 580; two of them got Na2SeO4 in doses of 4ppm within their drinking water. The molars of the lower jaws of one of these group were brushed with a tooth-paste containing NaF. The molars of the other group were treated with a paste, serving as a placebo. Two other groups were provided each with the paste containing fluorine and the paste serving as a placebo, but without selenium in their drinking water. Furthermore 20 animals were used as a control group. The analysis of variance showed the widely known protective effect of the fluorine by the statistically significant different distribution of the caries. Comparing those two groups, whose drinking water contained Na2SeO4 with the two other groups whose molars were treated with the paste containing fluorine and the one serving as a placebo respectively, we may conclude, that Na2SeO4 has no influence on the protective effect of the fluorine. A comparison of the group treated with the paste serving as a placebo with the control group shows, that the tooth-brushing obviously has no great effect. In respect to the body weight at the end of the experiment one cannot demonstrate a clear effect using the linear contrast of SCHEFFE, and there also is no infleunce of selenium on the total water consumption, which coincides with the results of the first experiment. The two experiments have shown that Na2SeO4 is toxic in the doses applied...
用来自奥斯本-孟德尔品系的100只大鼠进行了两项实验，旨在证明含硒饮用水对龋齿的影响。这些动物被分成五个实验组，每组20只大鼠。以亚硒酸钠的形式在饮用水中添加不同剂量的硒进行测试，同时也与含氟化钠的牙膏联合使用。其毒性将通过饮水量、死亡率，尤其是实验结束时动物的体重来确定。在第一个实验中，三组动物在饮用水中分别接受1、5和10 ppm剂量的亚硒酸钠，并以致龋饮食斯蒂芬580作为食物。另外两组作为对照组，一组接受致龋饮食斯蒂芬580，另一组接受正常繁殖饮食。为评估龋齿进行的方差分析和谢费检验仅显示，接受致龋饮食的组与喂食阿尔特明R繁殖饮食的对照组在龋齿侵袭方面存在差异。接受5和10 ppm硒剂量的组中的40只动物中有9只在实验中死亡。硒组动物的饮水量和体重平均值低于其他组，这表明亚硒酸钠具有很强的毒性。然而，关于饮水量，无法确切确定这是否是致龋饮食中硒的作用。第二个实验的所有组都接受致龋饮食斯蒂芬580；其中两组在饮用水中接受4 ppm剂量的亚硒酸钠。其中一组动物的下颌磨牙用含氟化钠的牙膏刷牙。另一组用作为安慰剂的牙膏处理。另外两组分别使用含氟牙膏和作为安慰剂的牙膏，但饮用水中不含硒。此外，20只动物用作对照组。方差分析显示，由于龋齿分布在统计学上有显著差异，氟具有广为人知的保护作用。比较饮用水中含亚硒酸钠的两组与另外两组，其磨牙分别用含氟牙膏和作为安慰剂的牙膏处理，我们可以得出结论，亚硒酸钠对氟的保护作用没有影响。将用作为安慰剂的牙膏处理的组与对照组进行比较表明刷牙显然没有很大效果。关于实验结束时的体重，使用谢费线性对比无法证明有明显影响，并且硒对总饮水量也没有影响，这与第一个实验的结果一致。这两项实验表明，所应用剂量的亚硒酸钠具有毒性……
