Mihm U, Safarli K, Botzenhart K
Zentralbl Bakteriol Orig B. 1976 Aug;162(5-6):558-65.
The applicability of 2 colour reactions were tested for the monitoring of the working concentration of disinfectant solutions containing aldehyde or phenol. The red coloration of Schiff's acid solution (Schiff's reagent to aldehydes) can be used with limitations. The formation of a red dye by the reaction of phenols with 1-phenyl-2,3-dimethyl-4-amino-pyrazolone-(5) in the p-esence of alkali and potassium hexacyano-ferrate (III) is unequivocal and is not influenced by contaminations in the water. Evaluation is made in either case by comparison with standard dilutions of the respective commercially available preparation. The samplest procedure for this a visual colour comparison in test tubes with calibration spectrum. Pyrazolone dye can be analysed photometrically. Thus this colour reaction can be regarded as an analytic method of satisfactory accuracy. A frequent monitoring of the disinfectant concentrations by means of simple chemical procedures is expected to help limit the improper use of disinfectants and to check hospital infections.
测试了两种颜色反应在监测含醛或酚类消毒剂溶液工作浓度方面的适用性。席夫酸溶液（席夫试剂与醛类反应）的红色显色有一定局限性。在碱和铁氰化钾（III）存在下，酚类与1-苯基-2,3-二甲基-4-氨基吡唑啉酮-（5）反应形成红色染料的过程明确，且不受水中污染物影响。在这两种情况下，均通过与各自市售制剂的标准稀释液进行比较来评估。对此的采样程序是在带有校准光谱的试管中进行目视颜色比较。吡唑啉酮染料可用光度法分析。因此，这种颜色反应可被视为一种准确性令人满意的分析方法。通过简单的化学程序对消毒剂浓度进行频繁监测，有望有助于限制消毒剂的不当使用并检查医院感染情况。
