绿色荧光核酸染料和氧化铝膜过滤器在土壤和沉积物细菌快速落射荧光计数中的应用
Utility of green fluorescent nucleic acid dyes and aluminum oxide membrane filters for rapid epifluorescence enumeration of soil and sediment bacteria.
作者信息
Weinbauer M G, Beckmann C, Höfle M G
机构信息
GBF-National Research Center of Biotechnology, AG Microbial Ecology, D-38124 Braunschweig, Germany.
出版信息
Appl Environ Microbiol. 1998 Dec;64(12):5000-3. doi: 10.1128/AEM.64.12.5000-5003.1998.
Abstract
High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4',6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circumvented by using green fluorescent high-affinity nucleic acid dyes and aluminum oxide membrane filters. Due to the bright staining of cells, we recommend SYBR Green II as dye.
摘要
高背景荧光和非特异性染色妨碍了在45%的经测试土壤和沉积物样本中，使用4',6-二脒基-2-苯基吲哚（DAPI）和聚碳酸酯膜过滤器对细菌进行落射荧光计数。通过使用绿色荧光高亲和力核酸染料和氧化铝膜过滤器，可以规避总细胞数测定中的这些问题。由于细胞染色明亮，我们推荐使用SYBR Green II作为染料。
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