构建用于检测多氯联苯的生物发光报告菌株。
Construction of a bioluminescent reporter strain To detect polychlorinated biphenyls.
作者信息
Layton AC, Muccini M, Ghosh MM, Sayler GS
机构信息
The Center for Environmental Biotechnology, The University of Tennessee, Knoxville, Tennessee 37996-1605, USA.
出版信息
Appl Environ Microbiol. 1998 Dec;64(12):5023-6. doi: 10.1128/AEM.64.12.5023-5026.1998.
Abstract
A bioluminescent reporter strain, Ralstonia eutropha ENV307(pUTK60), was constructed for the detection of polychlorinated biphenyls by inserting the biphenyl promoter upstream of the bioluminescence genes. In the presence of a nonionic surfactant, which enhances the solubility of chlorinated biphenyls, bioluminescence was induced three- to fourfold over background by biphenyl, monochlorinated biphenyls, and Aroclor 1242. The minimum detection limits for these compounds ranged from 0.15 mg/liter for 4-chlorobiphenyl to 1.5 mg/liter for Aroclor 1242.
摘要
通过将联苯启动子插入生物发光基因上游，构建了一株用于检测多氯联苯的生物发光报告菌株，即真养产碱菌ENV307(pUTK60)。在非离子表面活性剂（可提高氯化联苯的溶解度）存在的情况下，联苯、单氯联苯和Aroclor 1242诱导的生物发光比背景值高出三到四倍。这些化合物的最低检测限范围为：4-氯联苯为0.15毫克/升，Aroclor 1242为1.5毫克/升。
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本文引用的文献
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Science. 1990 Aug 17;249(4970):778-81. doi: 10.1126/science.249.4970.778.
2
Validation of genetically engineered bioluminescent surfactant resistant bacteria as toxicity assessment tools.经基因工程改造的抗生物发光表面活性剂细菌作为毒性评估工具的验证。
Ecotoxicol Environ Saf. 1999 Jun;43(2):222-8. doi: 10.1006/eesa.1999.1792.
3
Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase.通过联苯双加氧酶的定向进化增强多氯联苯的降解
Nat Biotechnol. 1998 Jul;16(7):663-6. doi: 10.1038/nbt0798-663.
4
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J Ind Microbiol Biotechnol. 1997 Oct;19(4):252-62. doi: 10.1038/sj.jim.2900454.
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