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通过变性梯度凝胶电泳监测不同接种物稀释度的富集培养物中细菌物种组成的变化。

Changes in bacterial species composition in enrichment cultures with various dilutions of inoculum as monitored by denaturing gradient gel electrophoresis.

作者信息

Jackson C R, Roden E E, Churchill P F

机构信息

Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama 35487, USA.

出版信息

Appl Environ Microbiol. 1998 Dec;64(12):5046-8. doi: 10.1128/AEM.64.12.5046-5048.1998.

DOI:10.1128/AEM.64.12.5046-5048.1998
PMID:9835607
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC90967/
Abstract

Denaturing gradient gel electrophoresis revealed changes in the bacterial species obtained from enrichment cultures with different inoculum dilutions. This inoculum dilution enrichment approach may facilitate the detection and isolation of a greater number of bacterial species than traditional enrichment techniques.

摘要

变性梯度凝胶电泳显示，从不同接种物稀释度的富集培养物中获得的细菌种类发生了变化。与传统富集技术相比，这种接种物稀释富集方法可能有助于检测和分离更多的细菌种类。