恶臭假单胞菌TVA8中三氯乙烯对tod操纵子的诱导作用。
Induction of the tod operon by trichloroethylene in Pseudomonas putida TVA8.
作者信息
Shingleton J T, Applegate B M, Nagel A C, Bienkowski P R, Sayler G S
机构信息
Center for Environmental Biotechnology, The University of Tennessee, Knoxville, Tennessee 37996, USA.
出版信息
Appl Environ Microbiol. 1998 Dec;64(12):5049-52. doi: 10.1128/AEM.64.12.5049-5052.1998.
Abstract
Bioluminescence, mRNA levels, and toluene degradation rates in Pseudomonas putida TVA8 were measured as a function of various concentrations of toluene and trichloroethylene (TCE). TVA8 showed an increasing bioluminescence response to increasing TCE and toluene concentrations. Compared to uninduced TVA8 cultures, todC1 mRNA levels increased 11-fold for TCE-treated cultures and 13-fold for toluene-treated cultures. Compared to uninduced P. putida F1 cultures, todC1 mRNA levels increased 4.4-fold for TCE-induced cultures and 4.9-fold for toluene-induced cultures. Initial toluene degradation rates were linearly correlated with specific bioluminescence in TVA8 cultures.
摘要
测定了恶臭假单胞菌TVA8中的生物发光、mRNA水平以及甲苯降解率与各种浓度的甲苯和三氯乙烯（TCE）之间的函数关系。TVA8对TCE和甲苯浓度的增加表现出生物发光反应增强。与未诱导的TVA8培养物相比，经TCE处理的培养物中todC1 mRNA水平增加了11倍，经甲苯处理的培养物中增加了13倍。与未诱导的恶臭假单胞菌F1培养物相比，经TCE诱导的培养物中todC1 mRNA水平增加了4.4倍，经甲苯诱导的培养物中增加了4.9倍。TVA8培养物中初始甲苯降解率与特定生物发光呈线性相关。
相似文献
1
Induction of the tod operon by trichloroethylene in Pseudomonas putida TVA8.恶臭假单胞菌TVA8中三氯乙烯对tod操纵子的诱导作用。
Appl Environ Microbiol. 1998 Dec;64(12):5049-52. doi: 10.1128/AEM.64.12.5049-5052.1998.
2
Kinetic analysis of a tod-lux bacterial reporter for toluene degradation and trichloroethylene cometabolism.用于甲苯降解和三氯乙烯共代谢的tod-lux细菌报告基因的动力学分析
Biotechnol Bioeng. 2000 Aug 5;69(3):256-65. doi: 10.1002/1097-0290(20000805)69:3<256::aid-bit3>3.0.co;2-2.
3
Quantification of toluene dioxygenase induction and kinetic modeling of TCE cometabolism by Pseudomonas putida TVA8.恶臭假单胞菌TVA8对甲苯双加氧酶诱导的定量分析及三氯乙烯共代谢的动力学建模
Biotechnol Bioeng. 2001 Dec;76(4):341-50. doi: 10.1002/bit.10083.
4
Pseudomonas putida B2: a tod-lux bioluminescent reporter for toluene and trichloroethylene co-metabolism.恶臭假单胞菌B2：一种用于甲苯和三氯乙烯共代谢的tod-荧光素酶生物发光报告菌。
J Ind Microbiol Biotechnol. 1997 Jan;18(1):4-9. doi: 10.1038/sj.jim.2900334.
5
Toluene dioxygenase expression correlates with trichloroethylene degradation capacity in Pseudomonas putida F1 cultures.甲苯双加氧酶表达与恶臭假单胞菌 F1 培养物中三氯乙烯降解能力相关。
Biodegradation. 2012 Sep;23(5):683-91. doi: 10.1007/s10532-012-9544-y. Epub 2012 Feb 19.
6
Gene components responsible for discrete substrate specificity in the metabolism of biphenyl (bph operon) and toluene (tod operon).负责联苯代谢（bph操纵子）和甲苯代谢（tod操纵子）中离散底物特异性的基因组件。
J Bacteriol. 1993 Aug;175(16):5224-32. doi: 10.1128/jb.175.16.5224-5232.1993.
7
Real-time reverse transcription PCR analysis of trichloroethylene-regulated toluene dioxygenase expression in Pseudomonas putida F1.实时逆转录 PCR 分析三氯乙烯调控假单胞菌 F1 甲苯双加氧酶表达。
J Environ Sci Health B. 2011;46(4):294-300. doi: 10.1080/03601234.2011.559877.
8
Aerobic TCE degradation by encapsulated toluene-oxidizing bacteria, Pseudomonas putida and Bacillus spp.包埋甲苯氧化菌（假单胞菌和芽孢杆菌）有氧降解三氯乙烯
Water Sci Technol. 2010;62(9):1991-7. doi: 10.2166/wst.2010.471.
9
Toluene-degrading bacteria are chemotactic towards the environmental pollutants benzene, toluene, and trichloroethylene.降解甲苯的细菌对环境污染物苯、甲苯和三氯乙烯具有趋化性。
Appl Environ Microbiol. 2000 Sep;66(9):4098-104. doi: 10.1128/AEM.66.9.4098-4104.2000.
10
Addition of aromatic substrates restores trichloroethylene degradation activity in Pseudomonas putida F1.添加芳香族底物可恢复恶臭假单胞菌F1中的三氯乙烯降解活性。
Appl Environ Microbiol. 2004 May;70(5):2830-5. doi: 10.1128/AEM.70.5.2830-2835.2004.
引用本文的文献
1
Fluoro-recognition: New in vivo fluorescent assay for toluene dioxygenase probing induction by and metabolism of polyfluorinated compounds.荧光识别：用于探测多氟化合物对甲苯双加氧酶的诱导作用及其代谢的新型体内荧光测定法。
Environ Microbiol. 2022 Nov;24(11):5202-5216. doi: 10.1111/1462-2920.16187. Epub 2022 Oct 17.
2
Genetically modified whole-cell bioreporters for environmental assessment.用于环境评估的转基因全细胞生物报告器。
Ecol Indic. 2013 May 1;28:125-141. doi: 10.1016/j.ecolind.2012.01.020.
3
Detection of organic compounds with whole-cell bioluminescent bioassays.采用全细胞生物发光生物测定法检测有机化合物。
Adv Biochem Eng Biotechnol. 2014;144:111-51. doi: 10.1007/978-3-662-43385-0_4.
4
The evolution of the bacterial luciferase gene cassette (lux) as a real-time bioreporter.细菌荧光素酶基因盒（lux）的进化作为实时生物报告器。
Sensors (Basel). 2012;12(1):732-52. doi: 10.3390/s120100732. Epub 2012 Jan 11.
5
Reconstructing the evolutionary history of nitrotoluene detection in the transcriptional regulator NtdR.重建转录调控因子 NtdR 中对硝基甲苯检测的进化历史。
Mol Microbiol. 2009 Nov;74(4):826-43. doi: 10.1111/j.1365-2958.2009.06904.x. Epub 2009 Oct 22.
6
Influence of nanophase titania topography on bacterial attachment and metabolism.纳米相二氧化钛表面形貌对细菌附着和代谢的影响。
Int J Nanomedicine. 2008;3(4):497-504. doi: 10.2147/ijn.s4399.
7
Effects of dichloroethene isomers on the induction and activity of butane monooxygenase in the alkane-oxidizing bacterium "Pseudomonas butanovora".二氯乙烯异构体对烷烃氧化细菌“布氏假单胞菌”中丁烷单加氧酶的诱导及活性的影响
Appl Environ Microbiol. 2005 Oct;71(10):6054-9. doi: 10.1128/AEM.71.10.6054-6059.2005.
8
Induction of methyl tertiary butyl ether (MTBE)-oxidizing activity in Mycobacterium vaccae JOB5 by MTBE.甲基叔丁基醚（MTBE）对母牛分枝杆菌JOB5中MTBE氧化活性的诱导作用
Appl Environ Microbiol. 2004 Feb;70(2):1023-30. doi: 10.1128/AEM.70.2.1023-1030.2004.
9
Characterization of the adaptive response to trichloroethylene-mediated stresses in Ralstonia pickettii PKO1.皮氏罗尔斯通氏菌PKO1中三氯乙烯介导的应激适应性反应的表征
Appl Environ Microbiol. 2002 Nov;68(11):5231-40. doi: 10.1128/AEM.68.11.5231-5240.2002.
10
Development and characterization of a green fluorescent protein-based bacterial biosensor for bioavailable toluene and related compounds.一种基于绿色荧光蛋白的用于检测生物可利用甲苯及相关化合物的细菌生物传感器的开发与特性研究。
Appl Environ Microbiol. 2002 Apr;68(4):1962-71. doi: 10.1128/AEM.68.4.1962-1971.2002.
本文引用的文献
1
Transformation capacities of chlorinated organics by mixed cultures enriched on methane, propane, toluene, or phenol.以甲烷、丙烷、甲苯或苯酚富集的混合培养物对氯化有机物的转化能力。
Biotechnol Bioeng. 1995 Mar 5;45(5):440-9. doi: 10.1002/bit.260450509.
2
Specific and quantitative assessment of naphthalene and salicylate bioavailability by using a bioluminescent catabolic reporter bacterium.利用生物发光代谢报告细菌对萘和水杨酸生物利用度进行特异性和定量评估。
Appl Environ Microbiol. 1992 Jun;58(6):1839-46. doi: 10.1128/aem.58.6.1839-1846.1992.
3
A chromosomally based tod-luxCDABE whole-cell reporter for benzene, toluene, ethybenzene, and xylene (BTEX) sensing.一种基于染色体的用于检测苯、甲苯、乙苯和二甲苯（BTEX）的tod-luxCDABE全细胞报告基因。
Appl Environ Microbiol. 1998 Jul;64(7):2730-5. doi: 10.1128/AEM.64.7.2730-2735.1998.
4
Pseudomonas putida B2: a tod-lux bioluminescent reporter for toluene and trichloroethylene co-metabolism.恶臭假单胞菌B2：一种用于甲苯和三氯乙烯共代谢的tod-荧光素酶生物发光报告菌。
J Ind Microbiol Biotechnol. 1997 Jan;18(1):4-9. doi: 10.1038/sj.jim.2900334.
5
Comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria.甲苯氧化细菌对三氯乙烯降解的影响因素比较
Appl Environ Microbiol. 1996 Mar;62(3):825-33. doi: 10.1128/aem.62.3.825-833.1996.
6
Trichloroethylene removal and oxidation toxicity mediated by toluene dioxygenase of Pseudomonas putida.恶臭假单胞菌甲苯双加氧酶介导的三氯乙烯去除及氧化毒性
Appl Environ Microbiol. 1994 Dec;60(12):4634-7. doi: 10.1128/aem.60.12.4634-4637.1994.
7
Induction of toluene oxidation activity in Pseudomonas mendocina KR1 and Pseudomonas sp. strain ENVPC5 by chlorinated solvents and alkanes.氯化溶剂和烷烃对门多萨假单胞菌KR1和假单胞菌属ENVPC5菌株甲苯氧化活性的诱导作用。
Appl Environ Microbiol. 1995 Sep;61(9):3479-81. doi: 10.1128/aem.61.9.3479-3481.1995.
8
Genomic sequencing.基因组测序
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5. doi: 10.1073/pnas.81.7.1991.
9
The aerobic pseudomonads: a taxonomic study.需氧假单胞菌属：一项分类学研究。
J Gen Microbiol. 1966 May;43(2):159-271. doi: 10.1099/00221287-43-2-159.
10
Degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with Pseudomonas putida F1.在恶臭假单胞菌F1的全细胞研究中，甲苯双加氧酶对三氯乙烯的降解作用。
Appl Environ Microbiol. 1988 Jul;54(7):1703-8. doi: 10.1128/aem.54.7.1703-1708.1988.
Zotero 插件