Shingleton J T, Applegate B M, Nagel A C, Bienkowski P R, Sayler G S
Center for Environmental Biotechnology, The University of Tennessee, Knoxville, Tennessee 37996, USA.
Appl Environ Microbiol. 1998 Dec;64(12):5049-52. doi: 10.1128/AEM.64.12.5049-5052.1998.
Bioluminescence, mRNA levels, and toluene degradation rates in Pseudomonas putida TVA8 were measured as a function of various concentrations of toluene and trichloroethylene (TCE). TVA8 showed an increasing bioluminescence response to increasing TCE and toluene concentrations. Compared to uninduced TVA8 cultures, todC1 mRNA levels increased 11-fold for TCE-treated cultures and 13-fold for toluene-treated cultures. Compared to uninduced P. putida F1 cultures, todC1 mRNA levels increased 4.4-fold for TCE-induced cultures and 4.9-fold for toluene-induced cultures. Initial toluene degradation rates were linearly correlated with specific bioluminescence in TVA8 cultures.
测定了恶臭假单胞菌TVA8中的生物发光、mRNA水平以及甲苯降解率与各种浓度的甲苯和三氯乙烯（TCE）之间的函数关系。TVA8对TCE和甲苯浓度的增加表现出生物发光反应增强。与未诱导的TVA8培养物相比，经TCE处理的培养物中todC1 mRNA水平增加了11倍，经甲苯处理的培养物中增加了13倍。与未诱导的恶臭假单胞菌F1培养物相比，经TCE诱导的培养物中todC1 mRNA水平增加了4.4倍，经甲苯诱导的培养物中增加了4.9倍。TVA8培养物中初始甲苯降解率与特定生物发光呈线性相关。
