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氯化溶剂和烷烃对门多萨假单胞菌KR1和假单胞菌属ENVPC5菌株甲苯氧化活性的诱导作用。

Induction of toluene oxidation activity in Pseudomonas mendocina KR1 and Pseudomonas sp. strain ENVPC5 by chlorinated solvents and alkanes.

作者信息

McClay K, Streger S H, Steffan R J

机构信息

Envirogen Inc., Lawrenceville, New Jersey 08648, USA.

出版信息

Appl Environ Microbiol. 1995 Sep;61(9):3479-81. doi: 10.1128/aem.61.9.3479-3481.1995.

Abstract

Toluene oxidation activity in Pseudomonas mendocina KR1 and Pseudomonas sp. strain ENVPC5 was induced by trichloroethylene (TCE), and induction was followed by the degradation of TCE. Higher levels of toluene oxidation activity were achieved in the presence of a supplemental growth substrate such as glutamate, with levels of activity of up to 86% of that observed with toluene-induced cells. Activity in P. mendocina KR1 was also induced by cis-1,2-dichloroethylene, perchloroethylene, chloroethane, hexane, pentane, and octane, but not by trans-1,2-dichloroethylene. Toluene oxidation was not induced by TCE in Burkholderia (Pseudomonas) cepacia G4, P. putida F1, Pseudomonas sp. strain ENV110, or Pseudomonas sp. strain ENV113.

摘要

门多萨假单胞菌KR1和假单胞菌属菌株ENVPC5中的甲苯氧化活性由三氯乙烯(TCE)诱导,诱导后TCE发生降解。在存在诸如谷氨酸等补充生长底物的情况下,可实现更高水平的甲苯氧化活性,其活性水平高达甲苯诱导细胞所观察到活性的86%。顺式-1,2-二氯乙烯、全氯乙烯、氯乙烷、己烷、戊烷和辛烷也可诱导门多萨假单胞菌KR1中的活性,但反式-1,2-二氯乙烯不能。洋葱伯克霍尔德菌(假单胞菌属)G4、恶臭假单胞菌F1、假单胞菌属菌株ENV110或假单胞菌属菌株ENV113中的甲苯氧化不会由TCE诱导。

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