恶臭假单胞菌甲苯双加氧酶介导的三氯乙烯去除及氧化毒性
Trichloroethylene removal and oxidation toxicity mediated by toluene dioxygenase of Pseudomonas putida.
作者信息
Heald S, Jenkins R O
机构信息
Department of Applied Biology and Biotechnology, De Montfort University, Scraptoft, Leicester, United Kingdom.
出版信息
Appl Environ Microbiol. 1994 Dec;60(12):4634-7. doi: 10.1128/aem.60.12.4634-4637.1994.
Abstract
Whole cells of Pseudomonas putida containing toluene dioxygenase were able to remove all detectable trichloroethylene (TCE) from assay mixtures. The capacity of cells to remove TCE was 77 microM/mg of protein with an initial rate of removal of 5.2 nmol/min/ng of protein. TCE oxidation resulted in a decrease in the growth rate of cultures and caused rapid cell death. Addition of dithiothreitol to assay mixtures increased the TCE removal capacity of cells by up to 67% but did not prevent TCE-mediated cell death. TCE induced toluene degradation by whole cells to a rate approximately 40% of that induced by toluene itself.
摘要
含有甲苯双加氧酶的恶臭假单胞菌全细胞能够从测定混合物中去除所有可检测到的三氯乙烯（TCE）。细胞去除TCE的能力为77微摩尔/毫克蛋白质，初始去除速率为5.2纳摩尔/分钟/纳克蛋白质。TCE氧化导致培养物生长速率下降并导致细胞快速死亡。向测定混合物中添加二硫苏糖醇可使细胞的TCE去除能力提高多达67%，但不能防止TCE介导的细胞死亡。TCE诱导全细胞甲苯降解的速率约为甲苯本身诱导速率的40%。
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