Villarreal D R, Sogal A, Ong J L
University of Texas Health Science Center at San Antonio, USA.
J Oral Implantol. 1998;24(2):67-73. doi: 10.1563/1548-1336(1998)024<0067:PAAORT>2.3.CO;2.
The purpose of this study was to investigate the effect of sintering different calcium phosphate (CaP) surfaces on protein adsorption and osteoblast cell response. As-received and sintered hydroxyapatite (HA) and brushite were used in this study. X-ray diffraction revealed a poorly crystallized HA structure for the unsintered HA and a highly crystallized HA for the sintered HA surfaces. A brushite-type structure was indicated on the unsintered brushite surfaces, whereas sintered brushite surfaces contained mixtures of different CaP phases. Using 1 mg/mL albumin solution, protein was suggested to selectively adsorb on the CaP surfaces. A statistically higher albumin adsorption was observed on unsintered HA (9.5 micrograms/mL) and unsintered brushite (50.1 micrograms/mL) surfaces compared to sintered HA (3.2 micrograms/mL) and sintered brushite (3.4 micrograms/mL) surfaces. In the in vitro study using osteoblast cells, no statistical responses were observed between cells cultured on sintered HA and sintered brushite after 8 days of incubation. However, statistical differences in osteocalcin and protein production were observed between the unsintered HA and unsintered brushite. In addition, statistical differences in protein production, alkaline phosphatase activity, and osteocalcin production were observed between sintered CaP and unsintered CaP surfaces. From the protein adsorption and cell responses observed in this study, it was concluded that CaP surfaces need to be fully characterized prior to implantation.
本研究的目的是调查烧结不同磷酸钙（CaP）表面对蛋白质吸附和成骨细胞反应的影响。本研究使用了原样的和烧结的羟基磷灰石（HA）及透钙磷石。X射线衍射显示，未烧结的HA呈现结晶度较差的HA结构，而烧结的HA表面则为高度结晶的HA结构。未烧结的透钙磷石表面显示为透钙磷石型结构，而烧结的透钙磷石表面含有不同CaP相的混合物。使用1mg/mL的白蛋白溶液时，蛋白质被认为会选择性地吸附在CaP表面。与烧结的HA（3.2微克/毫升）和烧结的透钙磷石（3.4微克/毫升）表面相比，在未烧结的HA（9.5微克/毫升）和未烧结的透钙磷石（50.1微克/毫升）表面观察到白蛋白吸附在统计学上更高。在使用成骨细胞的体外研究中，孵育8天后，在烧结的HA和烧结的透钙磷石上培养的细胞之间未观察到统计学上的反应差异。然而，在未烧结的HA和未烧结的透钙磷石之间观察到骨钙素和蛋白质产生的统计学差异。此外，在烧结的CaP和未烧结的CaP表面之间观察到蛋白质产生、碱性磷酸酶活性和骨钙素产生的统计学差异。从本研究中观察到的蛋白质吸附和细胞反应得出结论，CaP表面在植入前需要进行全面表征。
