Ong J L, Hoppe C A, Cardenas H L, Cavin R, Carnes D L, Sogal A, Raikar G N
University of Texas, Health Science Center at San Antonio, Department of Restorative Dentistry, San Antonio 78284-7890, USA.
J Biomed Mater Res. 1998 Feb;39(2):176-83. doi: 10.1002/(sici)1097-4636(199802)39:2<176::aid-jbm2>3.0.co;2-m.
The clinical success of dental implants is governed by implant surfaces and bone cell responses that promote rapid osseointegration and long-term stability. The specific objective of this study was to investigate osteoblast precursor cell responses to hydroxyapatite (HA) surfaces of different treatments. Since the nature of bone cell responses in vitro is influenced by the properties of HA ceramics, this study was divided into two components: a chemical and crystallographic characterization of the HA ceramics and an in vitro cell culture study. The sintered HA samples were observed to have the highest crystallite size as compared to the as-received HA and calcined HA samples. No differences in the surface roughness and chemical composition were observed among the sintered, calcined, and as-received HA surfaces. In concurrence with the X-ray diffraction, high resolution XPS resolution of Ca 2p also indicated a higher crystallinity on sintered HA samples as compared to the calcined and as-received HA samples. As indicated by increased alkaline phosphatase-specific activity, increased cell-surface and matrix-associated protein, and 1.25 (OH2) vitamin D3-stimulated osteocalcin production, a more differentiated osteoblast-like phenotype was observed on the sintered HA surfaces compared to the as-received HA and calcined HA surfaces. An increased osteoblast-like cell activity on the sintered HA surfaces suggested that the crystallite size of HA surfaces may play an important role in governing cellular response.
牙种植体的临床成功取决于种植体表面和骨细胞反应，这些反应促进快速骨整合和长期稳定性。本研究的具体目的是研究成骨细胞前体细胞对不同处理的羟基磷灰石（HA）表面的反应。由于体外骨细胞反应的性质受HA陶瓷性能的影响，本研究分为两个部分：HA陶瓷的化学和晶体学表征以及体外细胞培养研究。与原样HA和煅烧HA样品相比，观察到烧结HA样品具有最高的微晶尺寸。在烧结、煅烧和原样HA表面之间未观察到表面粗糙度和化学成分的差异。与X射线衍射结果一致，Ca 2p的高分辨率XPS分析也表明，与煅烧和原样HA样品相比，烧结HA样品具有更高的结晶度。与原样HA和煅烧HA表面相比，在烧结HA表面观察到更分化的成骨细胞样表型，表现为碱性磷酸酶特异性活性增加、细胞表面和基质相关蛋白增加以及1,25（OH）2维生素D3刺激的骨钙素产生增加。烧结HA表面上成骨细胞样细胞活性的增加表明，HA表面的微晶尺寸可能在控制细胞反应中起重要作用。
