Bajpai M, Gupta G, Jain S K, Setty B S
Division of Endocrinology, Central Drug Research Institute, Lucknow, India.
Andrologia. 1998 Nov;30(6):311-5. doi: 10.1111/j.1439-0272.1998.tb01176.x.
To assess the lipid metabolising potential of testicular germ cells undergoing meiosis, spermatocytes and spermatids were isolated from adult rat testis and purified by centrifugal elutriation followed by density gradient centrifugation. Seven key enzymes of lipid metabolism (namely beta-hydroxybutyrate dehydrogenase, carnitine acetyl transferase, ATP citrate lyase, hydroxyacyl-CoA dehydrogenase, glycerol 3-phosphate dehydrogenase, acetyl-CoA carboxylase and long chain acyl-CoA synthetase) were assayed in cell homogenates. The results indicated that germ cells possess the key enzymes for de novo synthesis and oxidation of fatty acids. The significant increase in activities of anabolic enzymes and decrease in activities of catabolic enzymes in post-meiotic germ cells indicated a shift in lipid metabolism towards fatty acid synthesis during meiosis. Long chain acyl-CoA synthetase activity was not detected in the two cell types. The study indicates a major reorganization of fatty acid turnover during meiosis with equilibrium shifting in favour of synthesis.
为评估进行减数分裂的睾丸生殖细胞的脂质代谢潜能，从成年大鼠睾丸中分离出精母细胞和精子细胞，并通过离心淘析随后进行密度梯度离心进行纯化。在细胞匀浆中测定了七种脂质代谢关键酶（即β-羟基丁酸脱氢酶、肉碱乙酰转移酶、ATP柠檬酸裂解酶、羟酰基辅酶A脱氢酶、甘油3-磷酸脱氢酶、乙酰辅酶A羧化酶和长链酰基辅酶A合成酶）。结果表明，生殖细胞拥有脂肪酸从头合成和氧化的关键酶。减数分裂后生殖细胞中合成代谢酶活性显著增加，分解代谢酶活性降低，表明减数分裂期间脂质代谢向脂肪酸合成转变。在这两种细胞类型中均未检测到长链酰基辅酶A合成酶活性。该研究表明减数分裂期间脂肪酸周转发生了重大重组，平衡向合成方向偏移。
