Linsalata M, Russo F, Notarnicola M, Berloco P, Di Leo A
Laboratory of Biochemistry, IRCCS Saverio de Bellis, Castellana Grotte, Bari, Italy.
Ital J Gastroenterol Hepatol. 1998 Oct;30(5):484-9.
Several studies have demonstrated increased gastric epithelial cell proliferation associated with Helicobacter pylori infection, which is reversed after bacterium eradication. Among the substances involved in cell proliferation and differentiation, polyamines are a group of polycations found in high concentrations both in normal and neoplastic cells.
Of the study were: a) to examine the influence of Helicobacter pylori infection on the polyamine profile in the gastric antrum and body, by comparing infected, to uninfected, patients, b) to evaluate the effect of successful and unsuccessful bacterium eradication on polyamine levels.
Twenty-six consecutive dyspeptic patients (20 Helicobacter pylori positive and 6 Helicobacter pylori negative) undergoing gastroscopy were enrolled. Polyamines were evaluated in antral and body biopsies by High Performance Liquid Chromatography.
Antral and body biopsies from Helicobacter pylori positive patients contained higher polyamine levels than those from Helicobacter pylori negative subjects. In Helicobacter pylori positive patients, the baseline polyamine levels were higher in the antrum than in the body. In Helicobacter pylori negative subjects, levels in the two stomach regions were similar. After therapy, polyamine levels decreased in patients with successful eradication, whereas these levels remained unchanged in patients in whom infection persisted.
These findings indicate enhanced antral cellular proliferation linked to the presence of Helicobacter pylori and add weight to the postulation of an association between Helicobacter pylori infection and increased risk of neoplastic changes in gastric antral mucosa. Differences in antral and body levels of polyamines may also be considered as a further indication of the different mucosal reactivity between the two regions of the stomach towards bacterial invasion.
多项研究表明，幽门螺杆菌感染会导致胃上皮细胞增殖增加，而在细菌根除后这种情况会逆转。在参与细胞增殖和分化的物质中，多胺是一组在正常细胞和肿瘤细胞中均高浓度存在的聚阳离子。
本研究的目的是：a）通过比较感染患者与未感染患者，研究幽门螺杆菌感染对胃窦和胃体多胺谱的影响；b）评估成功和未成功根除细菌对多胺水平的影响。
纳入26例连续接受胃镜检查的消化不良患者（20例幽门螺杆菌阳性和6例幽门螺杆菌阴性）。通过高效液相色谱法评估胃窦和胃体活检组织中的多胺。
幽门螺杆菌阳性患者的胃窦和胃体活检组织中的多胺水平高于幽门螺杆菌阴性患者。在幽门螺杆菌阳性患者中，胃窦的基线多胺水平高于胃体。在幽门螺杆菌阴性患者中，两个胃区域的水平相似。治疗后，成功根除的患者多胺水平下降，而感染持续的患者多胺水平保持不变。
这些发现表明胃窦细胞增殖增强与幽门螺杆菌的存在有关，并进一步支持了幽门螺杆菌感染与胃窦黏膜肿瘤性改变风险增加之间存在关联的假设。胃窦和胃体多胺水平的差异也可被视为胃的两个区域对细菌入侵的黏膜反应性不同的进一步指标。
