Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor.
作者信息
Zhang Y, Spremulli L L
机构信息
Department of Chemistry, Campus Box 3290, University of North Carolina, Chapel Hill, NC 27599-3290, USA.
出版信息
Biochim Biophys Acta. 1998 Nov 26;1443(1-2):245-50. doi: 10.1016/s0167-4781(98)00223-1.
PMID:9838146
Abstract
The complete sequence of the cDNA for the coding region of human mitochondrial translational release factor 1 has been obtained from human EST clones and 5'RACE. This sequence has been analyzed to provide insights into the relationship between release factors from bacteria and from mammalian mitochondria. The complete cDNA for the human mitochondrial ribosome recycling factor has been assembled using EST clones. This factor has been expressed in Escherichia coli and purified as a His-tagged protein.
摘要
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