Babu P G, Selvan A, Christuraj S, David J, John T J, Simoes E A
Department of Clinical Virology, Christian Medical College Hospital, Vellore, India.
Indian J Exp Biol. 1998 Aug;36(8):758-62.
To determine whether bonnet monkeys are susceptible to infection and disease due to respiratory syncytial virus (RSV), 4 juvenile bonnet monkeys (Macaca radiata) were inoculated with RSV intratracheally and sacrificed at 3, 5, 7 and 9 days post infection. RSV was cultured from pre-autopsy broncheoalveolar lavage fluid from all 4 animals with a peak titre of virus on day 9. Serum RSV neutralizing antibody was present by day 7. Animals developed tachypnoea and chest retractions by 5th day post infection and 2 animals had lobular pneumonia on chest radiography. The pathological changes were of a bronchovascular inflammation, interstitial pneumonia and alveolitis, akin to that seen in humans. These findings show that bonnet monkeys can be infected with RSV, and can develop immune response and clinical and pathological changes similar to those seen in human infants with RSV disease. Thus intractracheal RSV inoculation of juvenile bonnet monkeys appears to be a good model to study pathogenesis of RSV disease.
为了确定帽猴是否易受呼吸道合胞病毒（RSV）感染并发病，对4只幼年帽猴（恒河猴）进行气管内接种RSV，并在感染后第3、5、7和9天处死。从所有4只动物尸检前的支气管肺泡灌洗液中培养出RSV，第9天病毒滴度达到峰值。到第7天出现血清RSV中和抗体。感染后第5天动物出现呼吸急促和胸廓回缩，胸部X光检查显示2只动物有小叶性肺炎。病理变化为支气管血管炎症、间质性肺炎和肺泡炎，与人类所见相似。这些发现表明帽猴可感染RSV，并可产生免疫反应以及出现与患RSV疾病的人类婴儿相似的临床和病理变化。因此，对幼年帽猴进行气管内RSV接种似乎是研究RSV疾病发病机制的良好模型。
