Chung J G, Tan T W, Tsai H Y, Hsieh W T, Chen G W, Lai J M
Department of Microbiology, China Medical College, Taiwan, Republic of China.
Drug Chem Toxicol. 1998 Nov;21(4):507-20. doi: 10.3109/01480549809002219.
This study was designed to assess the effects of aspirin on arylamine N-acetyltransferase (NAT) activities in the bacterium Klebsiella pneumoniae using high performance liquid chromatography to measure the acetylation of 2-aminofluorene (2-AF) with or without aspirin. Cytosols or suspensions of K. pneumoniae with or without specific concentrations of aspirin co-treatment showed different percentages of 2-AF acetylation. The data indicated that there was decreased NAT activity associated with increased levels of aspirin in K. pneumoniae cytosols and in intact bacteria. For the cytosol examination, the apparent values of Km and Vmax decreased 0.59- and 0.58-fold after co-treated with 40 microM aspirin, respectively, for 2-AF. For the intact bacteria examination, the apparent values of Km and Vmax decreased 0.60- and 0.67-fold after co-treated with 40 microM aspirin, respectively, for 2-AF. This report is the first demonstration to show that aspirin can decrease N-acetyltransferase activity in the bacterium K. pneumoniae.
本研究旨在利用高效液相色谱法测量在有或没有阿司匹林存在的情况下2-氨基芴（2-AF）的乙酰化作用，以评估阿司匹林对肺炎克雷伯菌中芳胺N-乙酰基转移酶（NAT）活性的影响。有或没有特定浓度阿司匹林共同处理的肺炎克雷伯菌的胞质溶胶或悬浮液显示出不同百分比的2-AF乙酰化。数据表明，在肺炎克雷伯菌胞质溶胶和完整细菌中，随着阿司匹林水平的升高，NAT活性降低。对于胞质溶胶检测，用40微摩尔阿司匹林共同处理后，2-AF的Km和Vmax表观值分别下降了0.59倍和0.58倍。对于完整细菌检测，用40微摩尔阿司匹林共同处理后，2-AF的Km和Vmax表观值分别下降了0.60倍和0.67倍。本报告首次证明阿司匹林可降低肺炎克雷伯菌中的N-乙酰基转移酶活性。
