Dubois V, Moret H, Lafon M E, Janvresse C B, Dussaix E, Icart J, Karaterki A, Ruffault A, Taoufik Y, Vignoli C, Ingrand D
Department of Virology, PELLEGRIN Hospital, Bordeaux, France.
J Neurovirol. 1998 Oct;4(5):539-44. doi: 10.3109/13550289809113498.
Progressive Multifocal Leukoencephalopathy (PML) is a severe demyelinating disease, which is rapidly fatal and is due to JC virus (JCV) infection, which especially occurs in HIV-infected patients. To investigate JCV pathophysiology and to evaluate the predictive value of JCV detection in blood, we looked for JCV DNA in leukocytes and plasma of 96 patients without any neurological symptoms and 109 patients with neurological diseases, among whom 19 were suffering from PML. JCV genome was detected in about 18% of all patients, i.e. 15.6% of patients with central nervous system disorders except PML, 13.5% of patients without neurological symptoms and significantly more often in PML patients (47.6%). Both leukocytes and plasma were tested; in plasma, JCV DNA was found in 36.1% of positive patients and in cells in 80.5%. Surprisingly in seven instances only the plasma contained JCV genome. One-year follow-up of these patients showed that the absence of JCV DNA in blood was associated with a very low probability of developing PML (negative predictive value=0.99).
进行性多灶性白质脑病（PML）是一种严重的脱髓鞘疾病，病情发展迅速，可导致死亡，由JC病毒（JCV）感染引起，尤其在HIV感染患者中发病。为了研究JCV的病理生理学并评估血液中JCV检测的预测价值，我们在96例无任何神经系统症状的患者以及109例患有神经系统疾病的患者（其中19例患有PML）的白细胞和血浆中寻找JCV DNA。在所有患者中，约18%检测到JCV基因组，即除PML外的中枢神经系统疾病患者中有15.6%，无神经系统症状的患者中有13.5%，而PML患者中检测到的比例显著更高（47.6%）。同时检测了白细胞和血浆；在血浆中，36.1%的阳性患者检测到JCV DNA，在细胞中这一比例为80.5%。令人惊讶的是，有7例仅在血浆中检测到JCV基因组。对这些患者进行的一年随访表明，血液中未检测到JCV DNA与发生PML的可能性极低相关（阴性预测值 = 0.99）
