Melnikov O R
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kiev.
Technol Health Care. 1998 Sep;6(2-3):131-7.
Comparative investigations were performed to study the effect of endogenous and exogenous N-nitrosodiethylamine on the dynamics of content variations of oxidized cytochrome P-450 and its isoforms in the monooxygenase system of rat liver. The variations of cytochrome P-450 contents in both cases were demonstrated to be of the same character correlating with hepatocarcinogenesis stages. Higher quantities of oxidized cytochrome P-450 and its isoforms with MM 52, 53, and 56 kDa in the rat liver when acted upon by NDEA precursors are seen as the precondition of enhancing the monooxygenase reaction of NDEA bioactivation and, as a result, of the carcinogenic effects. Ascorbic acid is assumed to block the synthesis of NDEA from its precursors giving use to a compound whose metabolism does not influence the activity of the monooxygenase system of liver cells.
进行了对比研究，以探讨内源性和外源性N-亚硝基二乙胺对大鼠肝脏单加氧酶系统中氧化型细胞色素P-450及其同工型含量变化动态的影响。结果表明，两种情况下细胞色素P-450含量的变化具有相同特征，且与肝癌发生阶段相关。当受到NDEA前体作用时，大鼠肝脏中氧化型细胞色素P-450及其52、53和56 kDa同工型的含量较高，这被视为增强NDEA生物活化的单加氧酶反应以及致癌作用的前提条件。据推测，抗坏血酸可阻止NDEA前体合成NDEA，生成一种其代谢不影响肝细胞单加氧酶系统活性的化合物。
