Frenkel E P, White J D, Galey C, Croy C
Am J Clin Pathol. 1976 Nov;66(5):863-9. doi: 10.1093/ajcp/66.5.863.
The present study delineates the application of the radioisotopic competitive-inhibition assay for the measurement of vitamin B12 in tissues. The extraction of endogenous B12 from tissues was shown to be simple and complete. Proportional dilution studies suggest that tissue factors do not interfere with the assay. Although some variability exists when multiple areas of an organ are sampled, the differences between B12 levels in tissues obtained from normal individuals and B12-deprived individuals are so wide that individual intra-organ variability is trivial, As this tissue B12 assay is similar to the widely utilized radioisotopic assays for serum B12, it is applicable for routine use.
本研究阐述了放射性同位素竞争抑制法在组织中维生素B12测量中的应用。从组织中提取内源性维生素B12的方法简便且完全。比例稀释研究表明，组织因子不会干扰该检测。尽管对一个器官的多个区域进行采样时存在一定变异性，但正常个体和维生素B12缺乏个体的组织中维生素B12水平差异很大，以至于器官内个体差异微不足道。由于这种组织维生素B12检测方法与广泛应用的血清维生素B12放射性同位素检测方法相似，因此适用于常规使用。
