Vardareli E, Saricam T, Koken T, Degirmenci I, Aral E, Erenoglu E
Osmangazi University Medical School, Department of Gastroenterology, Eskisehir, Turkey.
Hepatogastroenterology. 1998 Sep-Oct;45(23):1505-8.
BACKGROUND/AIM: In the present study our purpose was to investigate the effect of pentoxyfilline, that plays a role in microcirculation and tissue oxygenation, alone and in combination with an antioxidant vitamin E on tissue damage in the rat liver induced by ischemia-reperfusion.
Thirty-one albino rats were divided into four groups. Rats in group I (n= 7), group II (n= 8) and group III (n= 8) were given, respectively, pentoxyfilline (25 mg/kg), pentoxyfilline and vitamin E in combination (25 mg/kg and 50 mg/kg, respectively) and equal volume of saline solution intraperitoneally for 7 days. Rats in group IV (n= 8) served as controls and received no treatment. On day 7 ischemia was induced by cross-clamping the hepatic artery, portal vein and left branch of the biliary duct for 30 minutes. Malondialdehyde (MDA) and catalase activity were assessed in tissue sample, and the level of ALT was measured in serum obtained after reperfusion for 30 minutes. Histological examination of tissue sample was also carried out.
There was no significant difference in ALT level between three study groups. Group I and group II had significant lower MDA and catalase levels than those of group III. The results of histopathologic examination in group I and group II were better than that of group III.
Our findings suggested that the treatment of pentoxyfilline alone and in combination with vitamin E decreased liver damage induced by ischemia-reperfusion and that the effect of latter was more effective but the difference between the two treatment patterns was not statistically significant.
背景/目的：在本研究中，我们的目的是研究在微循环和组织氧合中起作用的己酮可可碱单独使用以及与抗氧化剂维生素E联合使用对大鼠肝脏缺血再灌注诱导的组织损伤的影响。
将31只白化大鼠分为四组。第一组（n = 7）、第二组（n = 8）和第三组（n = 8）的大鼠分别腹腔注射己酮可可碱（25 mg/kg）、己酮可可碱与维生素E联合使用（分别为25 mg/kg和50 mg/kg）以及等体积的盐溶液，持续7天。第四组（n = 8）的大鼠作为对照，不接受治疗。在第7天，通过夹闭肝动脉、门静脉和胆管左支30分钟诱导缺血。评估组织样本中的丙二醛（MDA）和过氧化氢酶活性，并在再灌注30分钟后获得的血清中测量ALT水平。还对组织样本进行了组织学检查。
三个研究组之间的ALT水平没有显著差异。第一组和第二组的MDA和过氧化氢酶水平显著低于第三组。第一组和第二组的组织病理学检查结果优于第三组。
我们的研究结果表明，单独使用己酮可可碱以及与维生素E联合使用均可减少缺血再灌注诱导的肝损伤，且后者的效果更显著，但两种治疗方式之间的差异无统计学意义。
