Uguralp S, Bay Karabulut A, Mizrak B
Department of Pediatric Surgery, Turgut Ozal Medical Center, Inonu University, Faculty of Medicine, Malatya, Turkey.
Eur J Pediatr Surg. 2005 Apr;15(2):107-13. doi: 10.1055/s-2004-821256.
Pentoxifylline (PTX) and vitamin E inhibit the release of superoxide and hydroxyl radicals, and PTX improves capillary flow and tissue oxygenation. This experimental study was designed to determine the effects of PTX and vitamin E in the ovary after unilateral ovarian ischemia reperfusion (I-R) in albino Wistar rats. A vascular clamp was placed on the left ovary for 4 hours in all groups except for the control group. Following this, in the ischemia (I) group bilateral ovariectomy was performed. Saline, PTX, vitamin E, and PTX plus vitamin E were infused 30 min before reperfusion in the reperfusion (R), pentoxifylline (P), vitamin E (E), and pentoxifylline plus vitamin E (PE) groups, respectively. After 4 hours of reperfusion, the ovaries were removed for biochemical and histologic examination. MDA levels of bilateral ovaries in the PE group were significantly lower than in the E group (p < 0.0033). NO levels of bilateral ovaries in the PE group were significantly lower than in the P and E groups (p < 0.0033). Massive hemorrhage was determined in the ipsilateral ovaries of the R group. Hemorrhage was minimal or moderate in the ipsilateral ovaries of other groups. The contralateral ovaries showed congestion in different degrees. The contralateral ovaries of the group PE and the bilateral ovaries of the control group showed no pathological changes. PTX and vitamin E given together seems to be more effective in reducing I-R injury in ovarian tissue compared to administration of PTX, or vitamin E alone. However, further studies are required to evaluate the effective dose and duration of PTX and vitamin E on bilateral ovaries.
己酮可可碱（PTX）和维生素E可抑制超氧化物和羟基自由基的释放，且PTX可改善毛细血管血流和组织氧合。本实验研究旨在确定在白化Wistar大鼠单侧卵巢缺血再灌注（I-R）后，PTX和维生素E对卵巢的影响。除对照组外，所有组的左侧卵巢均用血管夹夹闭4小时。此后，缺血（I）组进行双侧卵巢切除术。在再灌注（R）组、己酮可可碱（P）组、维生素E（E）组和己酮可可碱加维生素E（PE）组中，分别在再灌注前30分钟注入生理盐水、PTX、维生素E和PTX加维生素E。再灌注4小时后，取出卵巢进行生化和组织学检查。PE组双侧卵巢的丙二醛（MDA）水平显著低于E组（p < 0.0033）。PE组双侧卵巢的一氧化氮（NO）水平显著低于P组和E组（p < 0.0033）。R组同侧卵巢出现大量出血。其他组同侧卵巢的出血为轻度或中度。对侧卵巢出现不同程度的充血。PE组的对侧卵巢和对照组的双侧卵巢均未显示病理变化。与单独给予PTX或维生素E相比，联合给予PTX和维生素E似乎在减轻卵巢组织的I-R损伤方面更有效。然而，需要进一步研究来评估PTX和维生素E对双侧卵巢的有效剂量和持续时间。
