Reiche L, Willis C, Wilkinson J, Shaw S, de Lacharrière O
Dermatology Department, Amersham General Hospital, Bucks, UK.
Contact Dermatitis. 1998 Nov;39(5):240-3. doi: 10.1111/j.1600-0536.1998.tb05916.x.
In this study of 152 women, comparison of patch test responses between 2 irritants over 96 h at 2 symmetrical anatomical sites is studied. 2 irritants, each at 4 different concentrations (nonanoic acid (NAA) 80%, 40%, 20%, 10%; sodium lauryl sulfate (SLS) 3%, 2%, 1% and 0.5%) and using propan-1ol and 'water for injection' as the respective controls, were placed as 15 microl aliquots, soaked onto filter paper discs in Finn Chambers, on the volunteer's left and right lower back. The patches were removed at 47, and read at 48 and 96 h. Irritant reactions were evaluated for erythema and surface changes by degree and area affected. Statistical analysis of the results showed that erythema decreased with time for all concentrations of NAA, and at higher concentrations for SLS. Surface changes increased with time for SLS and at higher concentrations of NAA. There was no statistically significant difference comparing left and right sides. Traditionally in patch testing, reactions which fade after 48 h have been regarded as irritant rather than allergic. This study refutes that assumption. Data from our left to right comparisons, made in the same individuals at the same time, show that irritant reactions may be more reproducible than previously appreciated.
在这项针对152名女性的研究中，研究了两种刺激物在96小时内于两个对称解剖部位的斑贴试验反应的比较。两种刺激物，每种有4种不同浓度（壬酸（NAA）80%、40%、20%、10%；月桂醇硫酸酯钠（SLS）3%、2%、1%和0.5%），并分别使用丙醇和“注射用水”作为对照，以15微升的等分试样形式，浸泡在Finn Chambers的滤纸片上，贴于志愿者的左右下背部。在47小时时去除贴片，并在48小时和96小时时读取结果。通过红斑程度和受影响面积评估刺激反应的红斑和表面变化。结果的统计分析表明，所有浓度的NAA的红斑随时间减少，SLS在较高浓度时红斑也减少。SLS的表面变化随时间增加，NAA在较高浓度时表面变化也增加。左右两侧比较无统计学显著差异。传统上在斑贴试验中，48小时后消退的反应被视为刺激性而非过敏性反应。本研究反驳了这一假设。我们在同一时间对同一受试者进行的从左到右比较的数据表明，刺激性反应可能比以前认为的更具可重复性。
