Hirsch P, Ludwig W, Hethke C, Sittig M, Hoffmann B, Gallikowski C A
Institut für Allegemeine Mikrobiologie, Universität Kiel, Germany.
Syst Appl Microbiol. 1998 Aug;21(3):374-383. doi: 10.1016/s0723-2020(98)80047-7.
Aseptically collected sandstone and soil samples from the antarctic Dry Valleys were inoculated into oligotrophic media and incubated under low light intensities. A total of 41 Gram-negative isolates were obtained with reddish colonies spreading on agar. A sandstone isolate and four soil strains were characterized further. They were nearly identical in morphological, physiological, biochemical and chemotaxonomic properties. They produced large amounts of extracellular polymer and utilized for growth: glucose, saccharose, mannitol, sorbitol, L-aspartate, malate and acetate, but not D-ribose, adonitol, DL-alanine, glutamate, glycolate, lactate or succinate. All strains hydrolyzed gelatin, starch, casein, xylan, Tweens 80 or 60 and dead or living yeast cells, but not cellulose or pectin. Nitrate was not reduced, ethanol was not oxidized and acid was not produced from maltose, mannitol or dulcitol. Ammonia was not produced from peptone. They were strictly aerobic. Major fatty acids were n 16:1 d 9, n 16:1 d 11, n 17:1 d 11, and i 15:0. The strains contained the quinone MK-7 and phosphatidylethanolamine as the main phospholipid. The base ratio ranged from 55 to 61 mol% G+C. A 16S rRNA sequence analysis of strains AA-688 and AA-718 showed these to be identical and to represent a special phylogenetic group within the Cytophaga/Flavobacterium/Bacteroides major line of descent. Three soil strains labeled "Taxeobacter" Txc1, Txg1, and Txo1 (Reichenbach, 1992) belonged to the same group but had lower sequence similarities (<95%). Some of their characteristics were different from those of the antarctic strains: the utilization of C-compounds, hydrolysis of polymers, temperature tolerances, major fatty acids and base ratios. Txc1 and Txg1 may later have to be considered as members of this group, possibly on the species level, while Txo1 could represent a different related genus. It is concluded that the five antarctic strains represent a new genus and species for which the name of Hymenobacter roseosalivarius is proposed. The type strain is AA-718T (DSM 11622T).
将从南极干谷无菌采集的砂岩和土壤样本接种到贫营养培养基中，并在低光照强度下培养。共获得41株革兰氏阴性分离株，其红色菌落在琼脂上扩散。对一株砂岩分离株和四株土壤菌株进行了进一步鉴定。它们在形态、生理、生化和化学分类学特性上几乎相同。它们产生大量胞外聚合物，并利用以下物质生长：葡萄糖、蔗糖、甘露醇、山梨醇、L-天冬氨酸、苹果酸和乙酸盐，但不能利用D-核糖、阿东醇、DL-丙氨酸、谷氨酸、乙醇酸、乳酸或琥珀酸盐。所有菌株都能水解明胶、淀粉、酪蛋白、木聚糖、吐温80或60以及死的或活的酵母细胞，但不能水解纤维素或果胶。不还原硝酸盐，不氧化乙醇，不能由麦芽糖、甘露醇或卫矛醇产酸。蛋白胨不产生氨。它们严格需氧。主要脂肪酸为n 16:1 Δ9、n 16:1 Δ11、n 17:1 Δ11和i 15:0。这些菌株含有醌MK-7和磷脂酰乙醇胺作为主要磷脂。碱基比例范围为55至61 mol% G+C。对菌株AA-688和AA-718的16S rRNA序列分析表明，它们相同，代表噬纤维菌属/黄杆菌属/拟杆菌属主要谱系中的一个特殊系统发育群。三株标记为“Taxeobacter”的土壤菌株Txc1、Txg1和Txo1（Reichenbach，1992）属于同一组，但序列相似性较低（<95%）。它们的一些特性与南极菌株不同：碳化合物的利用、聚合物的水解、温度耐受性、主要脂肪酸和碱基比例。Txc1和Txg1以后可能不得不被视为该组的成员，可能在种的水平上，而Txo1可能代表一个不同的相关属。结论是，这五株南极菌株代表一个新属和新种，为此提出了玫瑰唾液杆菌（Hymenobacter roseosalivarius）的名称。模式菌株是AA-718T（DSM 11622T）。
